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It is concluded that the optimal time of insemination using liquid semen is 12 to 0 h before ovulation, and 4 to 0 h before ovulation using frozen semen.
Gotwals: Fresh chilled semen is best inseminated in 24 to 48 hrs. The shorter the time interval from collection to insemination increases the odds that the semen will have the motility needed for pregnancy. It is true some stud dogs semen will last for 7-10 days but it is less than ideal to collect that far in advance.
Frozen semen is considered to be less preferable than fresh and chilled semen, however due to advances in technology, the gap between fresh/chilled and frozen is shrinking and in fact, frozen semen from some stallions achieves better conception rates than their chilled semen.
Artificial Insemination of Cattle Step by Step Preparation. The AI process. Insertion of palpating arm. Insertion of the AI catheter. Moving the AI catheter in the tract. Bringing the catheter to the cervix. Inserting the catheter into the cervix. Passing the catheter through the annular rings of the cervix.
During the procedure Attaches a vial that contains a sample of healthy sperm to the end of a long, thin, flexible tube called a catheter. Places the catheter into the vagina, through the cervix and into the uterus. Pushes the sperm sample through the tube into the uterus. Removes the catheter, followed by the speculum.