Acta Directorio Ford
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How to fill out First Board Of Directors Meeting Minutes - Corporate Resolutions?
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Each of the four decarboxylase broths is inoculated with four drops of the prepared suspension. A 4 mm layer of sterile mineral oil is added to each of the tubes. The tubes are then incubated for 4 days at 35-37°C in ambient air. The tubes are then observed for color change at 24, 48, 72, and 96 hours.
If the inoculated medium is yellow, or if there is no color change, the organism is decarboxylase-negative for that amino acid. If the medium turns purple, the organism is decarboxylase-positive for that amino acid.
Suspend 9 g of Ornithine Decarboxylase Broth in 1000 ml of distilled water. Heat if necessary to dissolve the medium completely. Dispense in test tubes and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
The final results are then obtained by observing the tube at 48 hours. Change back to purple from yellow indicates a positive test for lysine decarboxylase . Failure to turn yellow at 24 hours or to revert back to purple at 48 hours indicates a negative result.
The medium used is ornithine decarboxylase broth. The medium is a nutrient broth to which 0.5% ornithine is added. An important component of the medium is a modest amount of glucose, necessary for the process to proceed.