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The number En highlights the difference between the robust average with its uncertainty and the reference value with its uncertainty (Table 4). The number En between both the results are in the range [-1; 1] for all the calibrated masses.
A statistical estimate, called the lower limit factor, LLF, is derived from the calculated standard deviation and represents the width of the band of these deviations about the basic curve with a probability of approximately 99 %.
In principle, the uncertainty quantifies any possible difference between the calibrated value and its reference base (which normally depends on reference standards).
The CMC is determined with a tensiometer by measuring the surface tension of a concentration series. With pure surfactants, the SFT is linearly dependent on the logarithm of the concentration over a large range. Above the CMC, the SFT is extensively independent of the concentration.
In order to be able to determine the calibration factor, a reference system is required. Applicable reference systems are different flow measurement systems such as, for example, a Coriolis flow meter. Another possibility would be to simply measure the fluid volume over time in ml/min or the use of scales.