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  • Technical Note No. 0210 Rev. 2.1 Nucleocounter Nc-200

Get Technical Note No. 0210 Rev. 2.1 Nucleocounter Nc-200

Technical Note No. 0210 Rev. 2.1NucleoCounter NC200 NucleoCounter NC200 Performance Qualification Instrument and Materials Specifications Purpose: To specify the instrument, test kit and cassettes.

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How to fill out the Technical Note No. 0210 Rev. 2.1 NucleoCounter NC-200 online

This guide provides users with a clear, structured approach to filling out the Technical Note No. 0210 Rev. 2.1 for NucleoCounter NC-200 online. By following the detailed steps outlined below, you will effectively complete the form to ensure proper performance qualification.

Follow the steps to complete the Technical Note No. 0210 Rev. 2.1 NucleoCounter NC-200.

  1. Begin by pressing the 'Get Form' button to access the form and open it in your preferred online editor.
  2. In the first section, provide the instrument type by selecting NucleoCounter® NC-200™ and entering the corresponding catalog number.
  3. Input the instrument serial number and the firmware version for accurate identification of the device.
  4. Record the software version along with details for the NC-200 PQ Kit, including its catalog number, expiry date, and lot number.
  5. For the zero count procedure, select the protocol from the options presented, execute the steps, and record whether the result is successful.
  6. Proceed to the bead count procedure, ensuring to follow the preparation steps for beads carefully to avoid procedural variations.
  7. Fill in the results for low, medium, and high bead counts in the form, calculating the average and CV% where required.
  8. Complete the summary section by indicating whether each procedure was successful, using 'OK/Yes' or 'Fail/No' options where prompted.
  9. Lastly, provide the necessary signatures along with the date and your name in the performed by and verified by sections.

Complete your documents online today to ensure accurate record-keeping and compliance.

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The NucleoCounter® NC-200™ system enables the user to perform automated cell counting of a broad range of mammalian. Application. The Via1-Cassette™, Reagent A100 and B used together with the NucleoCounter® NC-200™ can be used for determining of the viability and cell concentration of aggregating cells.

The NucleoCounter is a novel, portable cell counting device based on the principle of fluorescence microscopy. The present work establishes its use with animal cells and checks its reliability, consistency and accuracy in comparison with other cytometric techniques.

How Our Cell Count & Viability Assay Works. Our NucleoCounter® instruments use automated fluorescence cytometry to count cells and determine viability. They rely on fluorescent dyes, acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI), to identify live and dead cells and provide accurate cell counts.

The working range of the NucleoCounter ® NC- 200™ is 5x103 to 1x107 cells/ml in the tested solution, and the optimal range is 5x104 to 5x106 cells/ml.

The NucleoCounter® NC-200™ system enables the user to perform automated cell counting of a broad range of mammalian. The Via1-Cassette™, Reagent A100 and B used together with the NucleoCounter® NC-200™ can be used for determining of the viability and cell concentration of aggregating cells.

A Zero Count is performed to ensure that there is no dust or other foreign particles on the lenses or camera, giving an artificially high cell count.

The working range of the NucleoCounter ® NC- 200™ is 5x103 to 1x107 cells/ml in the tested solution, and the optimal range is 5x104 to 5x106 cells/ml.

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