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  • Expi293f Expression System Kit The Expi293 Expression System Facilitates Large-scale Transfection

Get Expi293f Expression System Kit The Expi293 Expression System Facilitates Large-scale Transfection

Expi293F Expression System Kit Learn More Package Contents Storage Conditions Required Materials Catalog Number A14635 Buy Now Quantities Expi293F Cells ExpiFectamine 293 Transfection Kit Expi293.

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How to fill out the Expi293F Expression System Kit online

Filling out the Expi293F Expression System Kit form is essential for facilitating large-scale transfection using this optimized system. This guide provides clear, step-by-step instructions to help you complete the process effectively.

Follow the steps to accurately complete the Expi293F Expression System Kit form.

  1. Click ‘Get Form’ button to obtain the form and open it in the editor.
  2. Review the contents of the Expi293F Expression System Kit, which includes Expi293F Cells, ExpiFectamine 293 Transfection Kit, Expi293 Expression Medium, and the Antibody Expressing Positive Control Vector. Ensure you have all components on hand before proceeding.
  3. Fill out the section detailing your catalog number, which is A14635 for the kit, and ensure the quantity of each component is accurately specified according to your experimental needs.
  4. Indicate the required storage conditions for each component, such as storing Expi293F Cells in liquid nitrogen and the other reagents at 2°C to 8°C.
  5. Complete any additional sections related to the timing and steps for transfection, ensuring to follow the recommended timings for thawing and recovery (2-4 days), subculturing (every 3-4 days), and transfecting (1-7 days).
  6. Review the guidelines for optimal cell growth, such as maintaining cell densities between 3-5 x 10⁶ cells/mL and ensuring cells are subcultured appropriately.
  7. Once all fields are completed, save your changes, download a copy for your records, or print the form for submission.

Complete your Expi293F Expression System Kit form online to start your large-scale transfection process efficiently.

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The resultant Expi293F cells possess an increased growth rate compared to FreeStyle 293F Cells (average doubling times of 21–24 hours vs. 25–27 hours, respectively), higher viable cell densities, and increased cell viability.

HEK293 cells require splitting every 2–3 days while the Expi293 cells only require splitting every 4–5 days, which also substantially reduces the amount of laboratory work required to maintain cell viability. The cell line also expresses proteins better than its predecessor [20].

The Gibco™ Expi293™ Expression System is a high-yield transient expression system based on suspension-adapted Human Embryonic Kidney (HEK) cells that are highly transfectable and that will grow with a doubling time of 24-25 hours during log phase growth to high density in Expi293™ Expression Medium.

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium.

The Gibco™ Expi293™ Expression System is a high-yield transient expression system based on suspension-adapted Human Embryonic Kidney (HEK) cells that are highly transfectable and that will grow with a doubling time of 24-25 hours during log phase growth to high density in Expi293™ Expression Medium.

The Expi293 Expression System brings together a high-expressing 293 cell line, a chemically defined serum–free culture medium, and a high-efficiency transfection reagent with specialized enhancers.

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium.

What's in the Expi293 Expression system? The Expi293 Expression System brings together a high-expressing 293 cell line, a chemically defined serum–free culture medium, and a high-efficiency transfection reagent with specialized enhancers.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Help Portal
Legal Resources
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232