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Get Expi293f Expression System Kit The Expi293 Expression System Facilitates Large-scale Transfection
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How to fill out the Expi293F Expression System Kit online
Filling out the Expi293F Expression System Kit form is essential for facilitating large-scale transfection using this optimized system. This guide provides clear, step-by-step instructions to help you complete the process effectively.
Follow the steps to accurately complete the Expi293F Expression System Kit form.
- Click ‘Get Form’ button to obtain the form and open it in the editor.
- Review the contents of the Expi293F Expression System Kit, which includes Expi293F Cells, ExpiFectamine 293 Transfection Kit, Expi293 Expression Medium, and the Antibody Expressing Positive Control Vector. Ensure you have all components on hand before proceeding.
- Fill out the section detailing your catalog number, which is A14635 for the kit, and ensure the quantity of each component is accurately specified according to your experimental needs.
- Indicate the required storage conditions for each component, such as storing Expi293F Cells in liquid nitrogen and the other reagents at 2°C to 8°C.
- Complete any additional sections related to the timing and steps for transfection, ensuring to follow the recommended timings for thawing and recovery (2-4 days), subculturing (every 3-4 days), and transfecting (1-7 days).
- Review the guidelines for optimal cell growth, such as maintaining cell densities between 3-5 x 10⁶ cells/mL and ensuring cells are subcultured appropriately.
- Once all fields are completed, save your changes, download a copy for your records, or print the form for submission.
Complete your Expi293F Expression System Kit form online to start your large-scale transfection process efficiently.
The resultant Expi293F cells possess an increased growth rate compared to FreeStyle 293F Cells (average doubling times of 21–24 hours vs. 25–27 hours, respectively), higher viable cell densities, and increased cell viability.
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