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User Protocol 482702 Rev. 22-May-2008 RFH Nitric Oxide Synthase Assay Kit Colorimetric Cat. No. 482702 Note that this user protocol is not lot-specific and is representative of the current specifications for this product.

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How to fill out the Nitric Oxide Synthase Assay Kit Colorimetric 482702 Protocol Form online

This guide is designed to assist users in accurately filling out the Nitric Oxide Synthase Assay Kit Colorimetric 482702 Protocol Form online. By following these clear instructions, you can ensure that all necessary information is correctly submitted.

Follow the steps to complete the protocol form effectively.

  1. Click ‘Get Form’ button to obtain the form and open it in your preferred editor.
  2. Begin by entering your personal information in the designated fields if applicable. This usually includes your name, contact information, and organization.
  3. Locate the section for entering assay kit details. Input the Cat. No., which is 482702, and any other relevant kit information as specified.
  4. Provide the sample preparations information. Include details such as sample volume and specific requirements for different sample types (e.g., urine, plasma, or tissue homogenates).
  5. Fill in the assay conditions, including parameters like stopping methods, controls, and linearity considerations as necessary for your experimental design.
  6. After completing the necessary fields, review your entries for accuracy and completeness. Ensure that all sections required are filled out.
  7. Once you have confirmed that the form is complete, save your changes. Depending on your need, you can download, print, or share the completed form.

Complete the form online to facilitate your research with the Nitric Oxide Synthase Assay Kit Colorimetric.

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iNOS functions as a nodal point of the development of inflammatory spinal comprised of iNOS → Sirt1 S-nitrosylation → acetylation (activation) of p65 NF-κB → iNOS, which, in turn, supposedly contributes to burn-induced resistance and muscle wasting. iNOS is a major target gene of NF-κB.

iNOS and nNOS are soluble and found predominantly in the cytosol, while eNOS is membrane associated.

In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 μU of NOS activity.

The expression of the inducible nitric oxide synthase (iNOS) is one of the direct consequences of an inflammatory process.

Therefore, iNOS is an important marker of M1 macrophage activation. Despite increasing evidence that, in addition to its killing effect on pathogens, iNOS is involved in the regulation of immune responses, the importance of iNOS in controlling M1 macrophage differentiation is not fully understood.

Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in a variety of samples.

The Nitric Oxide Assay Kit is for quantitative determination of nitrite and nitrate in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540 nm.

A considerable number of human diseases have an inflammatory component, and a key mediator of immune activation and inflammation is inducible nitric oxide synthase (iNOS), which produces nitric oxide (NO) from l-arginine.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232