Get Pipette Calibration Worksheet 2020-2023

2:31 3:50 The most critical factors affecting precision are the calibration. Environment repeatable proceduresMoreThe most critical factors affecting precision are the calibration. Environment repeatable procedures and the personal skills of the calibrator.

The formula for calculating the volume dispensed by the pipette is V = w * Z where w is the weight of the water, Z is the conversion factor based on the density of the water, and V is the calculated volume of how much water was dispensed.

2:31 3:50 The most critical factors affecting precision are the calibration. Environment repeatable proceduresMoreThe most critical factors affecting precision are the calibration. Environment repeatable procedures and the personal skills of the calibrator.

Tolerance = (Measured Value - Expected Value)/Expected Value. In the above case the Tolerance is (75.1-75.0) / 75 = 0.13%. Tolerance is measurement of accuracy. Typically it is defined or specificed by the manufacturer of the device in question.

Finally, calculate accuracy by using the equation A = 100 x Vavg/V0, where A is the accuracy of the pipette, Vavg is the average calculated volume and V0 is the theoretical volume you tried to dispense. If the accuracy value lies in the 99-101% range, the pipette is considered normal and calibrated.

Use the formula V = w * Z to calculate the volume dispensed by the pipette. W is the weight of the distilled water. Z is the “conversion factor based on the density of the water” and V is the calculated volume of dispensed water.

The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh exactly 0.001 g using a high-precision balance.

Within pipette calibration there are five widely used grades of pipettes, all of which have specific guidelines and requirements regarding use, testing, maintenance, and measurement. The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette.