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The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma, and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma, and free cortisol in saliva and urine.

Principle of the Assay The Cortisol ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal rabbit antibody directed against the cortisol molecule.

An attached sensor detects cortisol using engineered strands of DNA, called aptamers, which are designed so that a cortisol molecule will fit into each aptamer like a key fits a lock. When cortisol attaches, the aptamer changes shape in a way that alters electric fields at the surface of a transistor.

Studies show this the saliva test is about 90% accurate in diagnosing Cushing syndrome. You'll do it at night, before you go to bed. That's because cortisol levels tend to be lowest between 11 p.m. and midnight. A high cortisol level near midnight can signal a disorder.

Cortisol can be measured in the blood, urine, saliva or a combination. Your healthcare provider will tell you which test they recommend for you. Blood test: In an office, clinic or lab, a healthcare provider inserts a thin needle into a vein in your arm. The needle collects a small sample of blood into a tube.

This assay is based on the competitive binding technique in which cortisol present in a sample competes with a fixed amount of alkaline phosphatase-labeled cortisol for sites on a mouse monoclonal antibody. During the incubation, the antibody becomes bound to the goat anti-mouse antibody coated onto the microplate.

If a person believes they have high or low cortisol levels, they may wish to take a cortisol test. Usually, these tests take place at a medical practice. However, several at-home cortisol tests are available to purchase. A person can take these tests at home by providing a urine, blood, or saliva sample.