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INTRODUCTION TO LABORATORY CRYOGENICS By M. N. Jirmanus, Ph.D. Janis Research Company, Inc. 2 Jewel Drive Wilmington, MA 01887 USA Tel: +1 978 6578750 Fax: +1 978 6580349 Email: sales janis.com www.janis.com.

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In a vacuum-insulated sample environment that uses liquid cryogens, a cryostat employs helium, nitrogen, or a mechanical cooler to cool a sample's temperature. Depending on the boiling point of the cryogen used, base temperatures of 77 K (nitrogen) or <4 K (helium) can be reached.

The cryostat is the instrument to freeze the tissue and also to cut the frozen tissue for microscopic section. The rapid freezing of the tissue sample converts the water into ice. The firm ice within the tissue acts as embedding media to cut the tissue.

Liquid helium, because of its low boiling point, is used in many cryogenic systems when temperatures below the boiling point of nitrogen are needed. A convenient way to cool many kinds of apparatus is to submerge them in liquid helium or liquid nitrogen.

Closed-cycle cryostats consist of a chamber through which cold helium vapour is pumped. An external mechanical refrigerator extracts the warmer helium exhaust vapour, which is cooled and recycled.

Helium is often used as a cooling agent, because it does not solidify under extreme temperatures. In fact, this gas doesn't become a liquid until -450 degrees Fahrenheit! Thus, it is used as a cooling agent by cooling down the superconductive magnetic coils, an integral component of an MRI system.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232