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JBC Papers in Press. Published on September 22, 2000 as Manuscript M006538200 Identification and cloning of a PAI1 mRNA binding protein Identification and cDNA cloning of a novel RNAbinding protein.

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cDNA (short for copy DNA; also called complementary DNA) is synthetic DNA that has been transcribed from a specific mRNA through a reaction using the enzyme reverse transcriptase. While DNA is composed of both coding and non-coding sequences, cDNA contains only coding sequences.

They also use cDNA libraries to identify genes that are expressed differently in different types of tissues or at different developmental stages. Libraries of cDNA molecules provide snapshots of gene activity, because only those genes that are actually expressed and transcribed into mRNA molecules can be cloned.

cDNA is complementary DNA that is synthesized from mRNA (messenger RNA). It is useful for cloning and expressing specific genes. Scientists can also create cDNA libraries, enabling them to study sequences specific to a given tissue and develop shareable databases.

cDNA clones are copies of mRNAs. Construction of cDNA clones involves the synthesis of complementary DNA from mRNA and then inserting a duplex copy of that into a cloning vector, followed by transformation of bacteria (Figure 3.6. 1).

In molecular biology, complementary DNA (cDNA) is synthesised from an RNA template in a reaction catalysed by the enzyme reverse transcriptase (RTase). cDNA synthesis is the first step in many molecular biology workflows, such as gene expression studies using real-time PCR.

One such technique is cDNA cloning. The principle behind this technique is that an mRNA population isolated from a specific developmental stage should contain mRNAs specific for any protein expressed during that stage. Thus, if the mRNA can be isolated, the gene can be studied.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232