Get Pet 16b Vector

pET-28a is a commonly used fusion protein type prokaryotic high-efficient expression vector, which contains resistance gene. Expression is induced by T7 RNA polymerase provided by the host cell. Host bacteria: It is recommended to clone E. coli DH5a or TOP10 as the recipient bacteria; use E.

The pET vector exists as a low copy number plasmid in host E. coli, which reduces leaky expression before induction. The vector utilizes the T7lac promoter system for strong and tightly controlled gene expression.

pET vector is an expression vector used for the expression of recombinant protein in E. coli.

The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.

pET vector is an expression vector used for the expression of recombinant protein in E. coli. This vector system utilizes the T7 lac promoter system for controlled gene expression. It is also called as Bacterial Recombinant Protein Vector.

Overview. The pET-16b vector carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB046). The pET Vectors are supplied as purified plasmid DNA (10 µg).

pET plasmid (plural pET plasmids) Any of a family of vectors for protein expression where the expression of the insert DNA is controlled by the T7 promoter, a phage promoter.

Salient features of pET28a, design flaws and improved designs a Genetic elements present in pET28a include the φ10 (T7) promoter and the lac operator, as well as the translation initiation region (TIR) encompassing the Shine–Dalgarno (SD) sequence, a spacer and the first five codons of the coding sequence.