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MiSeq? System User Guide FOR RESEARCH USE ONLY ILLUMINA PROPRIETARY Part # 15027617 Rev. F November 2012 This document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"),.

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ing to Illumina, MiSeq reagent kit v3 ensures double the output of a single run (3.3–15 Gb) compared to kit v2 (0.54–8.5 Gb)20. We validate this by determining the total number of (on-)target reads for both the v3 and v2 kit using the AMPure XP purification method.

MiniSeq From the Manage Instrument screen, select System Check. ... Add 40 ml deionized water to the wash cartridge in the reservoir equivalent to position 40 on the prefilled reagent cartridge. Load consumables as follows: ... Select Load. Select Next to start the system check.

MiSeq Reagent Kit v2 Supports 6–10 pM loading concentration. Follow the appropriate denature and dilute protocol depending on the procedure used during library prep.

HiSeq and MiSeq platforms are among the most widely used platform to study microbial com- munities. But the two platforms differ in the length and amount of reads. MiSeq can run 600 cycles to produce 200 million 300-bp reads, on the other hand, HiSeq 2500 can run 500 cycles to produce 120 million 250 bp.

The MiSeq System offers the first DNA-to-data sequenc- ing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment (Figure 1).

Illumina sequencing adapters and dual-index barcodes are then added to the generated amplicons during PCR using the Nextera XT DNA Index Kit. MiSeq V2 kits offer sequencing capacity up to 15M reads/run.

A 150-cycle run takes approximately 24 hours.

† A quality score (Q-score) is a prediction of the probability of an error in base calling....Quality Scores. † MiSeq Reagent Kit v2MiSeq Reagent Kit v3> 90% bases higher than Q30 at 2 × 25 bp> 70% bases higher than Q30 at 2 × 300 bp> 80% bases higher than Q30 at 2 × 150 bp> 75% bases higher than Q30 at 2 × 250 bp1 more row

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Living Trust
Name Change
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Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
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Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
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DocHub
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Call us now toll free:
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As seen in:
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© Copyright 1997-2025
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