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MiSeq? System User Guide FOR RESEARCH USE ONLY ILLUMINA PROPRIETARY Part # 15027617 Rev. F November 2012 This document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"),.

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How to use or fill out the MiSeq System User Guide (15027617) - Illumina online

This guide provides clear, step-by-step instructions on how to effectively fill out the MiSeq System User Guide, ensuring you understand each component and section. Designed to support both seasoned and novice users, the instructions aim for clarity and accessibility.

Follow the steps to complete the MiSeq System User Guide accurately.

  1. Begin by locating the MiSeq System User Guide (15027617) document and ensure you have a suitable environment to fill it out.
  2. Click the ‘Get Form’ button to obtain the form. This allows you to access the document for completion.
  3. Review the 'Introduction' section of the guide. Understand the features and intended use of the MiSeq system outlined.
  4. Proceed to the 'Getting Started' section. Familiarize yourself with required components, storage requirements, and flow cell types before setup.
  5. Fill out the 'Reagent Kit Information' section. Identify and note which MiSeq Reagent Kit you will be using, ensuring compatibility.
  6. Complete the 'MiSeq Software' section. Identify the version of MiSeq Control Software (MCS) in use, and ensure all software requirements are met.
  7. Refer to the 'Performing a Run' chapter to outline processes for the sequencing run, documenting any specific equipment or methods you plan to use.
  8. Finish by completing the 'Troubleshooting' section. Familiarize yourself with common issues and their resolutions to ensure a smooth operation.
  9. Once all sections are filled appropriately, save your changes or download a copy for your records. Options may also include printing or sharing the document.

Complete the MiSeq System User Guide online to enhance your sequencing experience and ensure accurate results.

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ing to Illumina, MiSeq reagent kit v3 ensures double the output of a single run (3.3–15 Gb) compared to kit v2 (0.54–8.5 Gb)20. We validate this by determining the total number of (on-)target reads for both the v3 and v2 kit using the AMPure XP purification method.

MiniSeq From the Manage Instrument screen, select System Check. ... Add 40 ml deionized water to the wash cartridge in the reservoir equivalent to position 40 on the prefilled reagent cartridge. Load consumables as follows: ... Select Load. Select Next to start the system check.

MiSeq Reagent Kit v2 Supports 6–10 pM loading concentration. Follow the appropriate denature and dilute protocol depending on the procedure used during library prep.

HiSeq and MiSeq platforms are among the most widely used platform to study microbial com- munities. But the two platforms differ in the length and amount of reads. MiSeq can run 600 cycles to produce 200 million 300-bp reads, on the other hand, HiSeq 2500 can run 500 cycles to produce 120 million 250 bp.

The MiSeq System offers the first DNA-to-data sequenc- ing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment (Figure 1).

Illumina sequencing adapters and dual-index barcodes are then added to the generated amplicons during PCR using the Nextera XT DNA Index Kit. MiSeq V2 kits offer sequencing capacity up to 15M reads/run.

A 150-cycle run takes approximately 24 hours.

† A quality score (Q-score) is a prediction of the probability of an error in base calling....Quality Scores. † MiSeq Reagent Kit v2MiSeq Reagent Kit v3> 90% bases higher than Q30 at 2 × 25 bp> 70% bases higher than Q30 at 2 × 300 bp> 80% bases higher than Q30 at 2 × 150 bp> 75% bases higher than Q30 at 2 × 250 bp1 more row

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Help Portal
Legal Resources
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
altaFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232