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  • 221 Transformation Of E. Coli With Pgal (blue Colony) - Edvotek

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The Biotechnology Education Company sed Revi nd a ated Upd EDVO-Kit # 221 Transformation of E. coli with pGAL (blue colony) Storage: See Page 3 for specific storage instructions Experiment Objective:.

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The names of two genes found on the plasmid used in the transformation experiment are AMPR gene ( Amphicillin Resistanct gene) and GFP gene ( Green Flouroscent Protein).

The pGLO plasmid contains a gene (bla or ampR) for a protein called β-lactamase, which hydrolyzes antibiotics that have a β-lactam ring and makes host cells resistant to compounds like .

The pGal plasmid contains the E. coli gene which codes for β-galactosidase. In the presence of artificial galactosides such as 5-Bromo-4 Chloro 3-indolyl-β-D-galactoside (X-Gal), pGal colonies appear blue when X-Gal is cleaved by β-galactosidase and forms a colored product.

If there are any genetically transformed bacterial cells they would be located on the +pGLO LB/amp and the +pGLO LB/amp/ara plates. This is because both these plates have the plasmid inside them which allows for the transformation.

Transformation of plasmid DNA into E. coli using the heat shock method is a basic technique of molecular biology. It consists of inserting a foreign plasmid or ligation product into bacteria.

The two most popular methods of bacterial transformation are (1) heat shock of chemically prepared competent cells (chemical transformation), and (2) electroporation of electrocompetent cells.

A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes.

If a resident E. coli strain is present in the gut, the invading strain evolves by rapid horizontal gene transfer (HGT), which precedes and outweighs evolution by accumulation of mutations. HGT is driven by 2 bacteriophages carried by the resident strain, which cause an epidemic phage infection of the invader.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232