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  • Guidelines For Ethidium Bromide Waste Management & Disposal ... - Web Utk

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Guidelines for Ethidium Bromide Waste Management & Disposal University of Tennessee-Knoxville 1. Background Ethidium bromide (3,8 diamino-5-ethyl-6-phenyl phenanthridinium bromide, dromilac, CAS.

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amounts of ethidium bromide (0.3-0.5 μg/ml) Agarose gels with trace amounts of ethidium bromide may be disposed of daily in double bag-lined containers (e.g., five- gallon pails). This waste is not considered a hazardous waste.

Handling. When pure ethidium bromide is used, handling should be performed in a fume hood wearing full protection clothing including a lab coat, closed-toe shoes, chemical resistant gloves and chemical safety goggles. can further reduce the risk of exposure, especially if the outer glove is replaced when contaminated.

Ethidium Bromide Disposal Procedure: Due to the toxicity of ethidium bromide, empty containers must also be collected and disposed of as hazardous waste. Do not discard any ethidium bromide down the sink drain or in the trash, unless the waste has been deactivated or filtered.

Concentrated ethidium bromide stock solutions and ethidium bromide in crystal/powder form will continue to be collected in satellite accumulation areas and disposed of as hazardous waste through an approved hazardous waste vendor.

Ethidium Bromide is a dye commonly used as a fluorescent tag when performing molecular biology techniques such as gel electrophoresis. This chemical is also a mutagen, teratogen and suspected carcinogen which must be handled as chemical waste, not a biohazardous waste.

Any solution that contains ethidium bromide AND is a hazardous chemical waste (flammable, toxic, reactive, corrosive, etc.) must be treated as hazardous chemical waste. Examples of hazardous chemical wastes include organic solvents or solutions containing heavy metals, cyanides or sulfides.

Ethidium Bromide Disposal Procedure: Do not discard any ethidium bromide down the sink drain or in the trash, unless the waste has been deactivated or filtered. Dry the gels in a chemical fume hood in an open container before disposing into a proper waste container.

Dispose of solid ethidium bromide through EHS as regular chemical waste. Aqueous solutions of ethidium bromide containing less than 1 microgram per milliliter (ug/ml) ethidium bromide and no other hazardous materials may be disposed down the drain with plenty of running water.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232