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Lating errors the stamp method will be used. A sample is plated on a non selective plate and let to grow for 16/18h, after what the colonies are stamped in the exact pattern on selective plates. All cells without a plasmid are either killed or prevented from growing (FRIEHS, 2004). Parameters like higher concentration of antibiotic (that could increase plasmid stability) and the presence of IPTG in the medium will also be analyzed in this study. In order to determine the presence of the insert.

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