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What do you store C18 columns in?

Always avoid storing the column in high aqueous phase (> 90% water) in order to prevent the phase collapse. The best way to store a C18 reverse phase column is to wash it with 50% Methanol (Methanol:Nanopure water) then store it in the same mix.

How should HPLC columns be stored?

HPLC columns can be stored in many solvents, but it is most important to store the column in a solvent mixture that will inhibit microbial growth. Short-Term HPLC Column Storage (up to 2 weeks): The column can be stored in the working mobile phase.

How should the columns be stored?

Normal phase columns should be stored in Hexane. Reversed phase stationary phases should be kept in a mixture of water / organic modifier (Acetonitrile/Methanol), free from any buffers or additives that might precipitate during storage.

How do you care for HPLC columns?

Cleaning HPLC Columns After using the column, it is always recommended to clean the column before storing it. The cleaning procedure typically involves using an isocratic/gradient wash that is closest to the last solvent system on the column , but replacing the buffer with HPLC grade water.

How do you store normal phase columns?

Silica-based normal phase columns should be stored with heptane or another inert solvent. Reversed phase columns are best stored in mixtures of organic compounds (acetonitril, methanol) and water, where the water content must not be higher than 50%.

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