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Columns can be divided into different types based on the shape of their cross-section. In this category, the most common types of columns are square/rectangular, circular columns, L-type columns, T-type columns and Y-type columns. These are further explained below.

Different Types of HPLC Columns Used in Analysis Normal Phase Columns. Reverse Phase Columns. Ion Exchange Columns. Size Exclusion Columns.

The types of chromatography useful in qualitative and quantitative analysis employed in USP procedures are column, gas, paper, thin-layer (including high-performance thin-layer chromatography), and pressurized liquid chromatography (commonly called high-pressure or high-performance liquid chromatography).

The term "liquid chromatography" (LC), as used in the compendia, is synonymous with high-pressure liquid chromatography and high-performance liquid chromatography. LC is a separation technique based on a solid stationary phase and a liquid mobile phase.

USP L Column Listing ColumnDescriptionL1Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10µm in diameterL2Octadecylsilane (C18) chemically bonded to silica gel of a controlled surface porosity, 30 to 50 µm in diameterL3Porous silica particles, 1.5 to 10µm in diameter35 more rows

Width @ Tangent (USP Resolution) is the width of the peak at the detected peak's inflection points. Width @ Tangent (USP Resolution) is defined by the width of the peak at the height percent defined by the Tangent Percent for USP Resolution parameter in the processing method.

The amount(s) of the minor component(s) (e.g. ? 50%) can be adjusted by ± 30 % relative. However a change in any component cannot exceed ± 10 % absolute. r Acetonitrile/Water could be adjusted to ± 12 % water (= 30 % of 40), but this exceeds the ± 10 % maximum absolute change.

Plate count is a theoretical number describing the separation efficiency of a chromatography column. In short, it is a measure an eluting compound's bandwidth at the time it elutes from a column, Equation 1. N = 16 (tr/Wb)2 Equation 1.

The shortcut USP comes from United States Pharmacopeial convention. Every kind of material or phase has a USP code. It is a general grouping for materials and the differences between materials with similar modifications are excluded. For example all C18 phases have the same USP column code.

The main difference between C8 and C18 column in HPLC is that the C8 column contains octyl carbon chain (C8)-bonded silica as the stationary phase, but the C18 column contains octadecyl carbon chain (C18)-bonded silica.