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Standard columns for reversed phase, normal phase, and ion exchange chromatography typically range from 3.9 to 4.6 mm internal diameter and 15, 25, and 30 cm in length. Standard columns for gel permeation chromatography (GPC) are 7.8 mm by 30 cm. Flow rates are typically 1 to 2 mL/min.

A Quick Look at Reversed Phase HPLCC18 HPLC columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage.C8 HPLC columns are used when less retention compared to a C18 is needed.More items...

The cost of HPLC columns can range anywhere between US $ 500 1000 depending on the specificity of applications.

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free OH functional groups, whereas BDS column contains deactivated OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

It may be possible to then flush the column, properly dispose of its contents, and recycle the hardware. If your columns are gently used, consider donating them to academic institutions in the developing world.

The cost of HPLC columns can range anywhere between US $ 500 1000 depending on the specificity of applications.

L8 An essentially monomolecular layer of aminopropylsilane (NH2) chemically bonded to totally porous silica gel support - 3 to 10 µm in diameter.

High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.

Different Types of HPLC Columns Used in Analysis Normal Phase Columns. Reverse Phase Columns. Ion Exchange Columns. Size Exclusion Columns.

Definition. C8 column refers to a type of column used in the reverse-phase chromatography, containing octylsilane as its stationary phase, while the C18 column refers to another type of column used in the reverse-phase chromatography, containing octadecylsilance as the stationary phase.