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Limitations of Resolution The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Aµm), the objects which are, at most, about 0.2 Aµm.

The resolution of an optical microscope is defined as the smallest distance between two points on a specimen that can still be distinguished as two separate entities.