05/30/18Paraffin Embedded Tissue Request FormPrincipal InvestigatorDateName: Department: Phone:BillingEmail:Clear FormREQUIREDRequest Number: Cost Center or Grant #*Fax:*Indicate all and allocationStudy.
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How to fill out the UNMC Paraffin Embedded Tissue Request Form online
Filling out the UNMC Paraffin Embedded Tissue Request Form online is an essential step for researchers requiring paraffin-embedded tissues. This guide provides a clear and concise overview to assist users in completing the form accurately and efficiently.
Follow the steps to complete the form online.
- Press the ‘Get Form’ button to access the form and open it for completion.
- Enter the principal investigator's details, including name, department, phone number, and email. Ensure all required fields are completed.
- In the billing section, provide the request number, cost center or grant number, and fax number as needed.
- Fill in the study contact information, including name, email, department, phone, and fax. Indicate if the billing address is the same as the principal investigator.
- Enter the mailing address, ensuring to include street, city, state, zip code, and phone number.
- In the request details section, list all de-identified case IDs from the NECARES report. Specify the type of tissue required, tumor/lesion details, and any control tissue if applicable.
- Indicate the number of unstained slides required and tissue core diameter, if necessary. Specify any special slide requests.
- Select the sections thickness and note the processing time. If a rush order is required, note that the price will double.
- Indicate if a pathologist review or advice is required, and provide specifics if applicable.
- Determine if you will utilize the Tissue Service Facility services and indicate the need to expedite the processing.
- Take note of any necessary PTB staff notes and fill in de-identified block IDs.
- For users filling out for the first time or annually, provide the IRB number and a summary of how the tissue will support the scientific purpose of the research.
- Enter the study title if submitted to the IRB, and complete the order completion section with details of material pick-up.
- Finally, save the changes, download a copy, print the form, or share it as needed.
Complete your document online today!
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How do you extract DNA from paraffin-embedded tissue?
To extract DNA from paraffin-embedded tissue, you typically need to remove the paraffin and resuspend the tissue in a suitable solution. Specialized extraction kits are available to simplify this process. If you have questions regarding this procedure, the UNMC Paraffin Embedded Tissue Request Form can provide valuable information and support.
What does paraffin do to tissues?
Paraffin acts as a protective medium for tissues, preventing degradation during storage. The embedding process allows for easier slicing into thin sections, which is vital for microscopic analysis. To manage your paraffin needs, begin with the UNMC Paraffin Embedded Tissue Request Form to streamline your request.
How do you prepare tissue for paraffin embedding?
Preparing tissue for paraffin embedding involves several steps: fixation, dehydration, clearing, and infiltration. Each step is crucial for preserving tissue architecture and ensuring successful embedding. You can simplify the preparation process by using the UNMC Paraffin Embedded Tissue Request Form for guidelines and further assistance.
What are the disadvantages of paraffin embedding?
While paraffin embedding is common, it has disadvantages, such as potential difficulty in extracting nucleic acids compared to fresh tissue. Additionally, the embedding process can alter certain cellular components, which might affect specific assays. Addressing these concerns is easy with the UNMC Paraffin Embedded Tissue Request Form, as it guides proper sample handling.
What is formalin-fixed paraffin-embedded tissue samples?
Formalin-fixed paraffin-embedded tissue samples are specimens that have been stabilized with formalin and then embedded in paraffin wax. This method allows long-term storage and easy sectioning for analysis. Utilizing the UNMC Paraffin Embedded Tissue Request Form can help you acquire these important samples efficiently.
How to fix tissue for paraffin embedding?
To fix tissue for paraffin embedding, ensure you use a fixative like formalin to stabilize the cells and preserve details. The tissue should be submerged in fixative for a specified duration, depending on the tissue type and size. Remember that the UNMC Paraffin Embedded Tissue Request Form is essential for initiating this process smoothly.
Why are tissues embedded in paraffin?
Tissues are embedded in paraffin to preserve their structure for microscopic examination. This process helps maintain the integrity of cellular details, allowing pathologists to make accurate diagnoses. The UNMC Paraffin Embedded Tissue Request Form plays a critical role in streamlining requests for these crucial samples.
How do you store paraffin-embedded tissue?
To store paraffin-embedded tissue, keep the samples in a cool, dry place away from direct sunlight. It is important to avoid extreme temperature fluctuations to maintain tissue integrity. When you need paraffin-embedded samples, the UNMC Paraffin Embedded Tissue Request Form can help you obtain them in optimal condition.
How do you prepare paraffin-embedded tissue sections?
To prepare paraffin-embedded tissue sections, tissues are first fixed, dehydrated, cleared, and then infiltrated with molten paraffin. Once cooled, the paraffin solidifies, and thin sections can be cut using a microtome. You can simplify this process by requesting your samples with the UNMC Paraffin Embedded Tissue Request Form.
What is paraffin-embedded tissue?
Paraffin-embedded tissue consists of biological samples that have been fixed in formalin and infiltrated with paraffin wax. This process provides stability and allows for detailed histological analysis. When you utilize the UNMC Paraffin Embedded Tissue Request Form, you access well-prepared samples crucial for accurate studies.
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