Get Guidelines For Beginning A Protein Expression Project - Research Chop

Filling out the Guidelines for Beginning a Protein Expression Project form is an essential step for initiating your protein expression project. This guide will help you navigate the process clearly and effectively.

Follow the steps to successfully complete your protein expression request.

1. Click ‘Get Form’ button to access the Protein Expression Request form and open it in your document editor.
2. Provide detailed information in the form, ensuring that both you and your principal investigator (PI) approve the submission before proceeding.
3. Attach an electronic copy of any relevant references that support your project.
4. Indicate specific information required, including antibiotic selection, the starting position of your gene, promoter details, primer sequences, unique restriction sites, and any affinity or epitope tags.
5. Provide verification of your gene, such as agarose gel results or DNA sequencing, as this evidence is necessary to begin your project.
6. If you're inexperienced in gene construction, consider seeking advice. The cloning process should preferably be conducted in the PI's laboratory due to costs and time involved.
7. If you have a construct ready, contemplate adding affinity tags for better purification and detection. Review the common tags and sequences provided in the guidelines.
8. Understand the pricing structure for the project, which includes charges for consumables and labor, and be prepared to receive an estimated cost before starting.
9. Finally, submit 2-5 µg of purified plasmid, labeling the tube correctly and ensuring you witness its placement in the designated storage area.
10. Once you have completed all fields, save your changes. You may have the option to download, print, or share the form if required.

Begin your protein expression project by completing the online guidelines now.