Get Sample Preparation Guidelines For Protein Identification - Research Chop

This guide provides clear instructions for completing the Sample Preparation Guidelines for Protein Identification at Research Chop. By following these steps, you can ensure that your sample preparation process is thorough and compliant with the necessary guidelines.

Follow the steps to accurately complete the sample preparation form.

1. Press the ‘Get Form’ button to obtain the sample submission form, which will open in your default digital document editor.
2. Begin by scheduling a consultation with the facility director, Mike Rosenblatt, to receive detailed guidance on the submission process.
3. Prepare your unknown protein sample, typically obtained from an immunoprecipitation or pull-down assay, and run it on a 1-D gel.
4. For larger scale preparations, consider running a 2-D gel or fractionating your sample using 2-D liquid chromatography.
5. After running the gel, excise the appropriate bands that correspond to your target proteins.
6. Follow the 'in-gel digest' protocol to prepare your samples for mass spectrometry analysis. Ensure that samples are free of salt and detergent.
7. Specify which protease will be used for digestion; options include trypsin, Lys-C, or Arg-C.
8. If multiple proteases are needed, indicate your preferences on the form.
9. Submit the completed sample submission form along with your prepared samples to the facility.
10. Once submitted, expect results back from the facility staff as soon as possible.
11. Finally, you can choose to save changes, download, print, or share the completed form as needed.

Complete your sample preparation documentation online today!