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EARCH AND DEVELOPMENT COMMAND Fort Detrick, Frederick, Maryland 21702-5012 Contract No. DAMD17-86-C-6055 Kansas State University Manhattan, Kansas 66506 Approved for public release: distribution A w The findings of this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents. DTIC ELECTE JUL319U SECURITY CLASSIFICATION OF THIS PAGE Form Approved REPORT DOCUMENTATION PAGE REPORT SECURITY CLASSIFICATION l. 0M8 No.

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In basic research labs, biologists often use DNA cloning to build artificial, recombinant versions of genes that help them understand how normal genes in an organism function.

Their ability to replicate independently makes plasmid a cloning vector in the recombinant DNA technology for transferring and manipulating genes. Many antibiotic-resistant genes in bacteria are present in plasmids.

Plasmids are used to transfer the information from one cell to another,i.e., transfer of important genes (e.g., they may confer resistance of particular, antibiotics to their bacterial cells), enabl to metabolise a nutrient, which normally a bacteria is unable to. It also helps in conjugation of bacteria.

Generally, scientists use plasmids to manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize plasmids across a wide range of applications.

General gene cloning steps: Isolation and preparation of the source DNA that you want to clone. Preparation of the cloning vector. Combining the vector and DNA fragments suitably so they form the recombinant DNA molecule. Introducing this recombinant DNA (vector + insert) into the host recipient.

Introduction. Molecular cloning refers to the isolation of a DNA sequence from any species (often a gene), and its insertion into a vector for propagation, without alteration of the original DNA sequence.

Molecular cloning refers to the isolation of a DNA sequence from any species (often a gene), and its insertion into a vector for propagation, without alteration of the original DNA sequence.

The term cloning describes a number of different processes that can be used to produce genetically identical copies of a biological entity. The copied material, which has the same genetic makeup as the original, is referred to as a clone.

Cloning is the process of generating a genetically identical copy of a cell or an organism. Cloning happens all the time in nature. In biomedical research, cloning is broadly defined to mean the duplication of any kind of biological material for scientific study, such as a piece of DNA or an individual cell.

The DNA molecules produced through the cloning techniques are used for many purposes, which include: DNA cloning can be used to make proteins such as with biomedical techniques. It is used to develop recombinant versions of the non-functional gene to understand the functioning of the normal gene.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232