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- Oligonucleotide Information Form - MR4
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To: MR4/American Type Culture Collection/10801 University Boulevard, Manassas, Virginia/20110-2209 USA Fax: 703-365-2774 * indicates required field * Depositor First name: Middle name: * Last name: Deposit date: * Depositor institution: MR4 registration number:.
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ORF FAQ
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Labels enable nucleic acid detection, yield information about its state, and can serve as a handle by which the nucleic acid and associated factors can be purified from a mixture. Radioactive phosphate is commonly added to the 5' or 3' end of an oligonucleotide post synthesis using enzyme-catalyzed reactions. End-labeling oligonucleotides with chemical tags after synthesis nih.gov https://pubmed.ncbi.nlm.nih.gov › ... nih.gov https://pubmed.ncbi.nlm.nih.gov › ...
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Oligonucleotides are small molecules 8–50 nucleotides in length that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA.
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For most uses, oligonucleotides are designed to base-pair with a strand of DNA or RNA. The most common use for oligonucleotides is as primers for PCR (polymerase chain reaction). Primers are designed with at least part of their sequence complementary to the 5' end of the sequence targeted for amplification.
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Among the diverse roles of RNA is the activity of long noncoding RNA (lncRNA) to regulate gene expression. A mechanism of increased transcription is to use oligonucleotides to block the binding of lncRNA to chromatin modifiers, which can suppress transcription. Oligonucleotide - an overview | ScienceDirect Topics sciencedirect.com https://.sciencedirect.com › topics › neuroscience sciencedirect.com https://.sciencedirect.com › topics › neuroscience
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Oligonucleotides, or oligos, are short single strands of synthetic DNA or RNA that serve as the starting point for many molecular biology and synthetic biology applications! From genetic testing to forensic research and next-generation sequencing, an oligo may very well be the starting point. What is an Oligo? - Thermo Fisher Scientific thermofisher.com https://.thermofisher.com › blog › behindthebench thermofisher.com https://.thermofisher.com › blog › behindthebench
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Oligonucleotides can be formed either enzymatically through cleavage of larger biomolecules or through targeted chemical synthesis. Oligonucleotide Synthesis | Solid Phase, Antisense, and DNA ... mt.com https://.mt.com › applications › L2_ReactionAnalysis mt.com https://.mt.com › applications › L2_ReactionAnalysis
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DNA is a very large molecule that contains millions of nucleotides! Oligonucleotides are short single or double-stranded fragments of DNA or RNA. In contrast to DNA, oligonucleotides usually contain from 13 to 25 nucleotides, but they can be larger as well. However, they rarely exceed 200 nucleotides.
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An oligosaccharide is an oligomer of monosaccharides (simple sugars). An oligonucleotide is a short single-stranded fragment of nucleic acid such as DNA or RNA, or similar fragments of analogs of nucleic acids such as peptide nucleic acid or Morpholinos.
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Keywords relevant to Oligonucleotide Information Form - MR4
- GenBank
- GC
- intron
- PlasmoDB
- manassas
- desalted
- ORF
- allele
- reagents
- depositor
- accession
- reagent
- originating
- CODING
- modifications
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