Get FIRST DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TOLPERISONE AND

There are two basic types of spectrophotometers. The single-beam spectrophotometer measures the relative light intensity before and after the test sample is introduced. The double-beam spectrophotometer compares the intensity of light between a reference light path and the substance being measured.

A derivative spectrophotometric method was developed for the assay of a ternary mixture of aspirin (ASP), paracetamol (PAR) and salicylic acid (SAL). The method is based on the use of the first and second derivatives of the ratio spectra and measurement at zero-crossing wavelengths.

A second-order derivative UV spectrophotometric method for determination of vitamin C [ascorbic acid (AA)] content in a variety of natural samples is described. The method is based on the measurement of a peak–baseline amplitude in the second derivative of the AA spectrum at 267.5 nm.

There are two main types of Spectrophotometer, namely the Single beam Spectrophotometer and the Double beam Spectrophotometer. These two Spectrophotometers can undertake different types of analysis and do not come with the same specifications.

Spectrophotometry (SP) It is a term that refers to the quantitative analysis of spectra to compare the relative absorption or emission of different wavelengths of light. Photometry is used to determine either the absolute amount or the relative amounts of two or more compounds or elements in a sample or set of samples.

These methods were absorptivity factor method and ratio subtraction method.

Among the different types of spectrophotometry, there are two primary methods employed; absorption spectrophotometry, which is concerned with the absorption of radiation and specific spectra of light, and Ultraviolet-Visible Range spectrophotometry, which is concerned with the reflectance of specific spectra of a given ...