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ReadyScript cDNA Synthesis Mix Catalog Number RDRT Storage Temperature 20 CTechnical BulletinProduct Description ReadyScript cDNA Synthesis Mix provides a sensitive and easytouse solution for twostep.

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How to fill out the ReadyScript cDNA Synthesis Mix online

Filling out the ReadyScript cDNA Synthesis Mix form online can streamline your process for obtaining this essential reagent. This guide offers detailed, step-by-step instructions to ensure you complete the form accurately and efficiently.

Follow the steps to successfully complete the ReadyScript cDNA Synthesis Mix form

  1. Press the ‘Get Form’ button to access the ReadyScript cDNA Synthesis Mix form and open it in your online document management system.
  2. Review the product information carefully, including the catalog number (RDRT) and storage temperature (−20 °C). Ensure you understand the components provided in the 5X concentrated master mix before proceeding.
  3. Fill in your personal information as the user of the product, such as your name, organization, and contact details, ensuring clarity for communication.
  4. Indicate the desired quantity of ReadyScript cDNA Synthesis Mix by selecting from the available options: RDRT-25RXN, RDRT-100RXN, or RDRT-500RXN. Make sure to select a quantity that meets your experimental needs.
  5. Read through and acknowledge any precautions and disclaimers provided in the form regarding product use and handling.
  6. Ensure that you understand the storage and stability information of the product included in the form, indicating any specific temperature requirements.
  7. Once all fields are filled out, review your entries to ensure that all information is accurate and complete, adjusting as necessary.
  8. Upon confirming all details, save your changes to the form and proceed to download, print, or share the document as required for your laboratory procedures.

Complete your document processing online today to ensure you have ready access to the ReadyScript cDNA Synthesis Mix for your research needs.

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Table 4. Recommended ranges of input RNA amounts in reverse transcription reactions. RNA templateRecommended rangeTotal RNA10 pg–5 μgPoly(A) RNA10 pg–0.5 μgSpecific RNA0.01 pg–0.5 μg

How much RNA is needed for RNA-seq? For standard RNA-seq library preparation, you should aim to start with between 100 ng to 1 µg of purified RNA. At least 500 ng purified total RNA is recommended by some core facilities.

The AccuPower RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA.

The useful range of input total RNA for the first strand cDNA template synthesis (reverse transcription) reaction is between 100ng and 5 µg.

There are three types of primers used to initiate reverse transcription depending on the RNA template and the downstream application (oligo(dT) primers, random primers, and gene-specific primers).

Ideally, the 260/280 ratio for RNA should be approximately 2.0 and the 260/230 ration should be 2.0-2.2. These ratios can be affected by pH so we are comfortable with all ratios over approximately 1.8. Ratios that differ significantly from that measure should undergo further purification.

Total RNA is routinely used in cDNA synthesis for downstream applications such as RT-(q)PCR, whereas specific types of RNAs (e.g., messenger RNA (mRNA) and small RNAs such as miRNA) may be enriched for certain applications like cDNA library construction and miRNA profiling.

The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for RT-PCR. It can be used with either sequence-specific primers, poly(dT)15 primers, or random primers, p(dN)6.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Help Portal
Legal Resources
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
altaFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232