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The remainder of the PCR then continues with the lower annealing temperature. For example, if you would normally use an annealing temperature of 54 °C, a touchdown PCR may start with an annealing temperature of 60 °C and drop down 1 °C /cycle over 10 cycles, and then continue at 50 °C for a further 30 cycles. Touchdown and Stepdown PCR - Bento Lab bento.bio https://bento.bio › resources › thoughts-notes-and-ideas bento.bio https://bento.bio › resources › thoughts-notes-and-ideas
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