Get Processing 2d Nmr Data With Mestrenova

This guide provides comprehensive instructions for users on how to successfully fill out the Processing 2D NMR Data With MestReNova form online. Follow each step to ensure proper processing and analysis of your 2D NMR data.

Follow the steps to fill out the form correctly

1. Click ‘Get Form’ button to obtain the form and open it in the editor.
2. Open the 2D data file by renaming the Bruker experiment number folder (1, 2, 3 ...) to HSQC, COSY, HMBC, ROESY, etc., before dragging it into MestReNova. Drag this folder into the main window of MestReNova. For Varian data, simply drag the filename.fid folder into the MestReNova window.
3. Set up the data processing method by selecting the Processing Template from the drop-down menu. Adjust parameters like window function and spectrum size according to the required settings for your specific 2D spectrum type. Ensure to apply appropriate settings for COSY, HSQC, HMBC, and others as specified.
4. Choose the contour display setup by right-clicking on the 2D spectrum and selecting Properties. Set preferences such as color palette and contour method. Adjust the number of contours and line width before saving the settings as default.
5. Open 1D spectra for traces. Ensure that the corresponding 1D spectra for the F2 and F1 dimensions are displayed properly. Reference the 1D spectra using the appropriate solvent peaks.
6. Perform 2D phase correction if necessary, particularly for HSQC data. Use manual correction to adjust phase errors carefully, ensuring minimal changes to maintain data integrity.
7. For detailed analysis, expand the region with crosspeaks by using the magnifying glass icon and selecting areas of interest.
8. Generate a contour plot by right-clicking on the spectrum and adjusting the contour plotting method. Fine-tune intensity thresholds to clearly display crosspeaks.
9. Reference the 2D spectrum by aligning it with the referenced 1D spectra to ensure accuracy in peak alignment.
10. Save your plotted data as a PDF file for convenient sharing or printing. Make sure to navigate to the correct folder and name the file appropriately.
11. Conduct analysis by using the crosshair tool to correlate 1D peaks with crosspeaks in the 2D display.
12. Utilize 1D slices from the 2D spectrum by selecting the desired crosspeaks and extracting them as new items for further examination.

Start processing your 2D NMR data online today!