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Real-time PCR allows for the detection of PCR amplification in the exponential growth phase of the reaction and is much more quantitative than traditional RT-PCR. Although a number of kits and reagents for RT-PCR and real-time PCR are commercially available, the basic principles are the same.

The main difference between RT-PCR and rapid PCR is the type of molecule that is being amplified. RT-PCR amplifies the RNA, whereas rapid PCR amplifies the DNA.

PCR (polymerase chain reaction): PCR (polymerase chain reaction): PCR (polymerase chain reaction) is a technique in molecular genetics that permits the analysis of any short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA.

RT-PCR: a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts: The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and. The amplification of a specific cDNA by the polymerase chain reaction (PCR).

The COVID-19 RT-PCR Test is a real-time reverse transcription polymerase chain reaction (rRT -PCR) test. The test can be run in a singleplex format (three individual assays for each of the three SARS-CoV-2 targets) or multiplexed into a single reaction (containing all 3 SARS-CoV-2 targets) and amplification set up.

Real-time PCR, also known as quantitative PCR (qPCR), combines PCR amplification and detection in a single step. Another technique known as reverse transcription polymerase chain reaction (RT-PCR) uses RNA as the nucleic acid starting template.

A laboratory method used to make many copies of a specific genetic sequence for analysis. It uses an enzyme called reverse transcriptase to change a specific piece of RNA into a matching piece of DNA. This piece of DNA is then amplified (made in large numbers) by another enzyme called DNA polymerase.

The COVID-19 RT-PCR Test is a real-time reverse transcription polymerase chain reaction (rRT -PCR) test. The test can be run in a singleplex format (three individual assays for each of the three SARS-CoV-2 targets) or multiplexed into a single reaction (containing all 3 SARS-CoV-2 targets) and amplification set up.