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2018 by Agilent TechnologiesAgilent CrossLab Compliance ServicesEQP Name:Standard EQP LC.01.96Service Type:OQCompany Name: Customer Name/Title: EQP Filename:Standard EQP LC.01.96.eqpEQP Release Date:June.

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Acceptable Tailing A new column is considered acceptable if the As value is 0.9 - 1.2 (0.9 indicates slight fronting). In practical terms, an As value below 1.5 is usually OK to work with, and up to As = 2.0 may be acceptable depending on the separation and resolution of the peaks.

How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated? Number of theoretical plates per column x 100/length of HPLC column (cm)= Number of theoretical Plates per m. Rss = (tr2 – tr1) / ((0.5 * (w1 + w2) Rs = (tR2 – tR1) / ((1.7 * 0.5 (w0.5,1 + w0.5,2))

fyi: Windows 10 support starts with the following software versions: OpenLab CDS Chemstation Edition C.

The EP recommends that the tailing factor should be between 0.8 and 1.5.

LABTips: How to Prevent Tailing Peaks in HPLC Buffer up and bring down the pH. ... Use end-capped/base-deactivated columns for analyzing basic compounds. ... Lose the dead volume. ... Filter, flush and backflush to bust blockages. ... Don't overload your column!

The tailing factor is determined by drawing a perpendicular line from the peak centre to the baseline of the peak. Then the peak width and the front half-width are measured for the peak at 5% of the height of the peak. The tailing factor is simply the entire peak width divided by twice the front half-width.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232