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ANALYTICAL BIOCHEMISTRY 209, 258266 (1993)A Spectral Study of the Charge Forms of Coomassie Blue G H. J. Chial, H. B. Thompson, and A. G. Splittgerber ' Chemistry Department, Gustavus Adolphus College,.

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Both Ponceau S and Coomassie Brilliant Blue stains are negatively charged solutions that bind to positively charged amino acid groups and non-polar protein regions.

Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent ("destaining"). This treatment allows the visualization of proteins as blue bands on a clear background.

This interference is based on the interaction of Coomassie Brilliant Blue G-250 (CBB) with this cationic detergent.

We found that the neutral ionic species of CBB binds to proteins by a combination of hydrophobic interactions and heteropolar bonding with basic amino acids.

It is an organic dye that makes complexes with basic amino acids, such as lysine, histidine, tyrosine, and arginine. The stain transfers an overall negative charge to the proteins allowing their separation from the polyacrylamide gel.

Definition: Coomassie Blue Stains This binding imparts overall negative charges to the proteins and facilitates their separation from SDS-PAGE gels based on their molecular weight.

The Coomassie Brilliant Blue G-250 dye has three forms: anionic (blue), neutral (green), and cationic (red). In an acidic environment, the red dye is converted into its blue form after binding to the protein of interest. If no protein binds to the dye, then the solution will remain brown.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Help Portal
Legal Resources
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232