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Capillary Isoelectric Focusing (cIEF) Analysis For the PA 800 Plus Pharmaceutical Analysis System Application GuideRUOIDV055862A February 2018This document is provided to customers who have purchased.

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Sciex - PA 800 Plus Pharmaceutical Analysis System The PA 800 Plus Pharmaceutical Analysis System is a robust analytical platform that provides characterization of product purity, charge heterogeneity, and glycan analysis, to help you with the development and quality control of therapeutic proteins.

In CE-SDS, proteins are denatured under reducing or nonreducing conditions in the presence of SDS and electrophoretically separated by molecular weight and hydrodynamic radius through a sieving polymer matrix. Acceptable performance of this method would yield protein peaks that are baseline resolved and symmetrical.

iCIEF separation is conducted by focusing isoforms in an ampholytic pH gradient through an applied electric field. Following the focusing step is a detection step, which in conventional CIEF would entail a chemical or pressure mobilization across a fixed UV detector 4,.

Imaged capillary isoelectric focusing (iCIEF) is a powerful technique used in many biopharmaceutical laboratories responsible for evaluating the charge heterogeneity of proteins. Bottlenecks occur in the manual preparation of different samples and variability of test results is introduced by the technician.

Capillary isoelectric focusing (cIEF) is a high-resolution analytical technique that allows the separation of protein/peptide mixtures, protein glycoforms and other charge variants, based on their isoelectric point (pI). cIEF is based on the principle of capillary gel electrophoresis (cGE).

Fast track characterization of multiple attributes of intact proteins. Accelerate your biotherapeutic development by directly coupling iCIEF (imaged capillary isoelectric focusing) charge variant analysis with high-resolution mass spectrometry detection for intact proteins.

The PA 800 plus application menu includes SDS-gel molecular weight analysis, charge heterogeneity analysis utilizing advanced capillary isoelectric focusing technology, and carbohydrate profiling for the assessment of glycoprotein microheterogeneity. Modular design provides for the simple interchange of detectors.

Isoelectric focusing (IEF) is a technique of exquisite resolution and high sensitivity. When applied to human biological fluids using conventional protein stains it is capable of detecting down to about 100 mg of protein/L. When combined with blotting and probing techniques it can get down to less than 1 mg/L.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232