Get Pa 800 Plus Pharmaceutical Analysis System Cief Application Guide

In CE-SDS, proteins are denatured under reducing or nonreducing conditions in the presence of SDS and electrophoretically separated by molecular weight and hydrodynamic radius through a sieving polymer matrix. Acceptable performance of this method would yield protein peaks that are baseline resolved and symmetrical.

iCIEF separation is conducted by focusing isoforms in an ampholytic pH gradient through an applied electric field. Following the focusing step is a detection step, which in conventional CIEF would entail a chemical or pressure mobilization across a fixed UV detector 4,.

Imaged capillary isoelectric focusing (iCIEF) is a powerful technique used in many biopharmaceutical laboratories responsible for evaluating the charge heterogeneity of proteins. Bottlenecks occur in the manual preparation of different samples and variability of test results is introduced by the technician.

Capillary isoelectric focusing (cIEF) is a high-resolution analytical technique that allows the separation of protein/peptide mixtures, protein glycoforms and other charge variants, based on their isoelectric point (pI). cIEF is based on the principle of capillary gel electrophoresis (cGE).

Fast track characterization of multiple attributes of intact proteins. Accelerate your biotherapeutic development by directly coupling iCIEF (imaged capillary isoelectric focusing) charge variant analysis with high-resolution mass spectrometry detection for intact proteins.

The PA 800 plus application menu includes SDS-gel molecular weight analysis, charge heterogeneity analysis utilizing advanced capillary isoelectric focusing technology, and carbohydrate profiling for the assessment of glycoprotein microheterogeneity. Modular design provides for the simple interchange of detectors.

Isoelectric focusing (IEF) is a technique of exquisite resolution and high sensitivity. When applied to human biological fluids using conventional protein stains it is capable of detecting down to about 100 mg of protein/L. When combined with blotting and probing techniques it can get down to less than 1 mg/L.