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Name: Restriction MappingDate: 1. Below is a restriction map for the plasmid pGEN101 (total length 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated.

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Restriction mapping has widely publicized applications. These include DNA fingerprinting, the detection of genetic defects and the sequencing of the human genome.

Steps in restriction mapping Preparation of DNA for restriction analysis. Restriction digestion of DNA. Separation of restricted DNA. Collecting data.

Restriction mapping exercises are designed to provide students with tangible experience in the abstract reasoning required for accurate interpretation of results. These exercises are meant to be carried out during electrophoresis of student samples.

A restriction map is a diagram that indicates the relative positions of restriction enzyme sites on a particular DNA sequence. To construct a map the DNA in question is cut with a variey of restriction enzymes both singly and in combination.

Restriction enzymes are traditionally classified into four types on the basis of subunit composition, cleavage position, sequence specificity and cofactor requirements.

Restriction mapping involves cutting DNA at various different recognition sites. Fragments are then separated and identified with gel electrophoresis. When a plasmid is cut by R.E only one strand of DNA is produced. Because of this. combinations of R.E are used to cut the plasmid into two fragments.

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airSlate Legal Forms, Inc.
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Content Takedown Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232