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Fluorophore selection guide for the Attune NxT Acoustic Focusing Cytometer UVIR 300Excitation laser400Emission filter (nm)ChannelRecommended dyes440/50VL1Alexa Fluor 405 Pacific BlueViability dyes.

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When selecting a panel of fluorophores for your flow cytometry panel, it is important to follow a few basic guidelines. First, it is important to understand your flow cytometer. You should know the number and types of lasers present, their excitation capabilities and how to set your filters.

Rhodamine. The spirolactam structure of rhodamine is nonfluorescent and colorless and exhibits high selectivity for metal ions; the rhodamine ring in its open form has strong fluorescence.

Select fluorophores with high extinction coefficients ( ε ) One defining factor of a fluorophore's brightness is its extinction coefficient (a measurement of the probability of absorbing a photon of light); the higher the value of the extinction coefficient the brighter the fluorophore.

Here the most important aspects to consider when you select a fluorescent dye: The extinction coefficient at the excitation wavelength should be as high as possible. ... The quantum yield should be as high as possible. ... The fluorescent dye should be photostable. ... Photochromic behavior. ... Live/dead cell permeability.

Select fluorophores with high extinction coefficients ( ε ) One defining factor of a fluorophore's brightness is its extinction coefficient (a measurement of the probability of absorbing a photon of light); the higher the value of the extinction coefficient the brighter the fluorophore.

isothiocyanate (FITC) is an organic fluorescent dye and probably one of the most commonly used in immunofluorescence and flow cytometry.

Selecting the Right Fluorophores for Flow Cytometry Experiments Understand your flow cytometer. ... Consider target abundance. ... Research fluorophore properties. ... Increase panel size with tandem dyes. ... Think about using calibration and compensation beads. ... Never underestimate the importance of compensation controls.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232