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GE Healthcare Firsttime use Sample recommendations Equilibrate the column for firsttime use or after longterm storage as follows: Instructions 71501788 AC a) Ion Exchange Columns b) Preparation Indepth.

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This guide provides a comprehensive overview of how to fill out the Mono Q 5 50 form online. Whether you are familiar with chromatography or new to the process, our clear instructions will support you in completing the form accurately.

Follow the steps to successfully complete the Mono Q 5 50 form.

  1. Click the ‘Get Form’ button to access the Mono Q 5 50 form and open it in your preferred editor.
  2. Begin by reading the introduction and purpose of the form to understand its significance and how to use it correctly.
  3. Fill out the personal or organizational details as requested. Ensure all information is accurate to avoid any delays.
  4. Provide specific information regarding your sample recommendations as outlined. Select appropriate buffers, flow rates, and temperatures relevant to your chromatography needs.
  5. Review each section carefully, including any instructions related to the equilibrium state and the subsequent steps for column usage.
  6. Finalize your entries by checking all fields for completeness and accuracy before saving.
  7. After completing the form, choose to save your changes, download, print, or share the form as needed.

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Wash with 2 column volumes of detergent in a basic or acidic solution, e.g. 0.1–0.5% non-ionic detergent in 0.1 M acetic acid (see Table 21 for recommended flow). Rinse with 5 column volumes 70% ethanol to remove residual detergent (see Table 21 for recommended flow).

Mono Q™ 5/50 GL and Mono S™ 5/50 GL are Tricorn™ high performance columns. The columns are pre-packed glass columns for high performance ion exchange chromatography of proteins, peptides, polynucleotides and other biomolecules.

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

Mono Q™ columns are strong anion exchange chromatography columns for protein analysis or small scale, high resolution polishing of proteins. Packed with MonoBeads™ Q strong anion exchange chromatography resin. Monodisperse 10 µm porous beads provide high resolution, reproducibility, and durability.

For removing resin residues, the most commonly used solutions are sodium hydroxide (NaOH) and sodium chloride (NaCl), or even hot water for injection (WFI).

If the column is to be stored for more than 2 days after use, wash the column with 4 column volumes (CV) of distilled water and then equilibrate with at least 4 CV of degassed 20% ethanol. Use a low flow rate, checking backpressure as the column equilibrates.

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