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PMB 185, 2010 Final Exam Initials Final Exam, 150 points Name PMB 185: Techniques in Light Microscopy Point value is in parentheses at the end of each question. Note: GFP green fluorescent protein;.

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An inverted phase-contrast microscope converts the direct light penetrating the specimens into the diffracted light. So, the specimen gets illuminated easily. On the other hand, normal standard microscopy uses beams of electromagnetic light to form the images of specimens under the microscope.

Confocal Microscopes The laser scans the sample using different patterns, and the image is assembled with the computer. The laser can penetrate the sample deeper than light from a bulb. The resulting image is a three-dimensional image of controlled depth of field.

The phase contrast microscope is used primarily in serological and glass examinations. Its principal use in serology is to observe cells in biological fluids or after reconstitution in aqueous mountants.

Phase-contrast microscopy is a technique used for gaining contrast in a translucent specimen without staining the specimen. One major advantage is that phase-contrast microscopy can be used with high-resolution objectives, but it requires a specialized condenser and more expensive objectives.

Phase contrast imaging with an electron microscope requires creating an analogous phase plate for electron waves. However, most designs rely on material placed near the electron beam, which can be charged or damaged by the electron beam, causing unwanted wavefront aberrations or resolution loss.

The major advantage of phase contrast is its ability to generate image contrast from materials that don't absorb light, including cells and tissues in culture. Thin, transparent, colorless samples can contain details so fine that, even if absorbent, don't show up well such as Cilia and flagella.

Different Types of Light Microscopy Dark Field Microscopy. Dark field vs bright field microscopy: Bright field microscopy uses the most basic and the common type of optical microscope. ... Fluorescence microscopy. ... Phase Contrast Microscopy. ... Differential Interference Contrast Microscopy. ... Confocal Microscopy. ... Polarized Microscopy.

Phase contrast is an optical contrast technique for microscopy which makes unstained structures in the cells of biological specimens visible. Cell structures that appear transparent with brightfield illumination can be viewed in high contrast and rich detail using phase contrast.

Phase-contrast microscopy is a technique used for gaining contrast in a translucent specimen without staining the specimen. One major advantage is that phase-contrast microscopy can be used with high-resolution objectives, but it requires a specialized condenser and more expensive objectives.

Notice that the unstained cells in the brightfield image are almost invisible against the background, whereas the cells in the phase-contrast image appear to glow against the background, revealing far more detail.

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Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232