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  • Protocol For Ribo-zero Rrna Removal Kit Plant Leaf

Get Protocol For Ribo-zero Rrna Removal Kit Plant Leaf

RiboZero rRNA Removal Kit* (Plant Leaf) Cat. No. RZPL11016 6 Reactions (Contains one box of Cat. No. RZPL11016 and one box of Cat. No. RZC1046) * Patent Pending www.epicentre.com Lit. # 319 6/2011.

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How to use or fill out the Protocol For Ribo-Zero RRNA Removal Kit Plant Leaf online

Filling out the Protocol For Ribo-Zero RRNA Removal Kit Plant Leaf ensures a smooth and accurate procedure for RNA processing. This guide provides clear instructions to walk users through each step of the process, tailored for both novice and experienced individuals.

Follow the steps to efficiently complete the protocol.

  1. Press the ‘Get Form’ button to retrieve the Protocol For Ribo-Zero RRNA Removal Kit Plant Leaf and access it in your preferred editor.
  2. Begin by reviewing the kit contents detailed in the document. Note the required components and their corresponding volumes to ensure you have everything necessary.
  3. Familiarize yourself with the introduction section of the protocol. This part outlines the advantages and process summary, providing important context for the steps that follow.
  4. Proceed to the preparation section. Follow the instructions related to maintaining an RNase-free environment and the proper handling of the total RNA samples.
  5. Move to the Ribo-Zero rRNA Removal Kit (Plant Leaf) procedure section. Follow the steps for preparing the Ribo-Zero microspheres, treating the total RNA sample, and the microsphere reaction.
  6. After the microsphere reaction, follow the purification steps using either ethanol precipitation or a column-based method as described in the document.
  7. Finally, ensure you quantify the yield and assess the quality of the rRNA-depleted sample according to the recommended techniques.
  8. Once all steps have been completed, you can save your changes, download the protocol, print it for physical records, or share it with your team as necessary.

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Streptavidin-coated paramagnetic beads are then added to the sample, capturing biotin-oligo-rRNA complexes. A magnet is then applied, allowing easy removal of bead-captured rRNA from the total RNA sample. The ribosomal RNA-depleted sample may then be concentrated depending on the downstream application.

SEQuoia RiboDepletion Kit is a stand-alone, post-library depletion kit that eliminate fragments derived from cytoplasmic ribosomal RNA (rRNA) and mitochondrial ribosomal RNA (mt rRNA) sequences from an RNA-Seq library. The ribosomal RNA is abundant, constituting 80–90% of total RNA.

Ribosomal RNA depletion promotes efficient detection of functionally relevant coding as well as non-coding transcripts through removal of highly abundant rRNA species.

The Ribo-Zero Gold Kit uses a proprietary method that is optimized for removal of all sizes of rRNA. Intact or degraded total RNA samples (100 ng to 5 μg) are mixed with the rRNA Removal Reagents in solution (25 minutes).

Ribo-Zero Plus uses DNA probes and enzymatic depletion to remove abundant transcripts from total RNA samples; whereas legacy Ribo-Zero kits use biotinylated RNA probes to pull down abundant transcripts with streptavidin beads (see Figure 1).

Ribo-Zero Plus uses DNA probes and enzymatic depletion to remove abundant transcripts from total RNA samples; whereas legacy Ribo-Zero kits use biotinylated RNA probes to pull down abundant transcripts with streptavidin beads (see Figure 1).

The Ribo-off rRNA depletion kit (Plant) is designed to deplete rRNA (including 5S, 18S and 25S rRNA) from total RNA of plant roots, seeds and leaves and to obtain mRNA and other non-coding RNA. This kit is suitable for removing rRNA from 1 μg - 5μg of plant total RNA.

Generally, one of two strategies is used to remove rRNA from total RNA, enrichment of mature polyadenylated (poly(A)) mRNA or targeted depletion of rRNA.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232