Get Xf96 Assay Preparation Checklist - Seahorse Bioscience

Filling out the XF96 Assay Preparation Checklist is essential for ensuring a successful assay run. This guide provides a clear and comprehensive overview of each section of the form, directing you on how to prepare effectively and accurately.

Follow the steps to complete the XF96 assay preparation checklist online.

1. Click the ‘Get Form’ button to acquire the XF96 Assay Preparation Checklist and open it in your preferred editor.
2. Enter the date at the top of the checklist to document when the assay preparation will occur.
3. Fill in the lab technician's name to identify who is responsible for the assay preparation.
4. Specify the cell type you will be using for the assay, as this is crucial for accurate results.
5. Review the status and tasks outlined under the 'Night before' section. Ensure you prepare the unbuffered assay medium with a pH of 7.4 at 37°C, hydrate the cartridge for a minimum of 12 hours in a non-CO2 incubator, and turn on the XF Analyzer and controller to allow them to reach 37°C.
6. Create or select a protocol for the assay from an existing template.
7. On the day of the assay, warm the assay medium to 37°C in a non-CO2 environment before proceeding.
8. Prepare injectable compounds using the previously warmed assay medium, adjusting the pH to 7.4 at 37°C.
9. Examine the cells under a microscope to ensure they are ready for the assay.
10. Replace the cell culture medium with the prepared assay medium to start the assay process.
11. Place the cells in the assay medium into a non-CO2 incubator for at least 30 minutes before the assay run.
12. Load the injectable compounds into the designated ports (A, B, C, D) of the cartridge.
13. Insert the cartridge and utility plate with calibration buffer into the XF Analyzer.
14. Run the calibration protocol from the template to ensure accuracy of the cartridge measurements.
15. After calibration, check the cells under the microscope again.
16. Upon successful calibration, load the cell culture plate into the XF Analyzer.
17. Continue executing the assay protocol as per the instructions provided in your protocol template.
18. Utilize the notes section at the bottom of the checklist to record any observations or important information.

Complete your XF96 Assay Preparation Checklist online to ensure a streamlined assay preparation process.