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XF96 Assay Preparation Checklist XF96 Assay Preparation Checklist Date: Lab Tech: Cell Type: Date: Lab Tech: Cell Type: Use this checklist when preparing to run an XF assay. Use this checklist when.

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For Oligomycin, 1.5 µM is recommended for most cell types, while for Rot/AA, 0.5 µM is recommended.

After gentle mixing, OCR and ECAR measurements are made multiple times. over time while up to four different drugs are injected sequentially into each well. Total assay time is typically 60 to 90 minutes.

The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. In mammalian cells, glycolysis and oxidative phosphorylation (OXPHOS) pathways provide the majority of cellular ATP.

The Agilent Seahorse Glycolysis Stress Test kit measures the capacity of the glycolytic pathway after glucose starvation. This is accomplished by driving cells toward glycolysis and assessing their ability to increase glycolytic activity to meet metabolic and bioenergetic demands.

Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate.

Only a small number of cells is required (4,000 – 500,000 per well). Measurements can be repeated multiple times for kinetic analysis. Measurements can be taken for minutes, hours, or even days.

Seahorse XF Glycolytic Rate Assay Kit Filter by For Use WithPart Number: 103346-100Description: Seahorse XFp Glycolytic Rate Assay KitList Price : 329.00 USDPart Number: 103344-100Description: Seahorse XF Glycolytic Rate Assay KitList Price : 689.00 USD2 more rows

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Content Takedown Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232