Get Cross-network Pbmc Processing Standard Operating Procedure - Hanc

Filling out the Cross-Network PBMC Processing Standard Operating Procedure - Hanc form is essential for proper documentation processing in specimen management. This guide will walk you through each section of the form to ensure accuracy and completeness.

Follow the steps to accurately complete the form online.

1. Click the ‘Get Form’ button to obtain the form and open it in the provided editor.
2. Begin by entering the specimen processing laboratory information in the designated section. Fill out the participant ID (PTID), visit details, protocol information, collection date, and specific time of collection.
3. Document the processing start date along with all relevant reagents and their manufacturers in the corresponding fields, ensuring to record the lot numbers for each reagent used.
4. Indicate the volume in mL for DMSO and FBS, as well as include data captured during processing, noting the sample type and blood condition. Make selections where applicable.
5. If protocol requires, indicate if plasma was harvested prior to PBMC processing. Note if any harvesting occurred by selecting yes or no.
6. Specify the usable whole blood volume in mL and indicate the processing method (e.g., frit barrier, manual over/under-lay, or buffy coat pooling). Provide the counting method and respective volumes as required.
7. Calculate cell yields and total cell numbers in the provided sections, ensuring to perform all necessary mathematical calculations accurately.
8. After completing all fields, save your changes, download a copy of the completed form, print it, or share it as required. Make sure to complete all LDMS entries as indicated at the end of the form.

Complete your form online today to streamline your PBMC processing and documentation!