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  • Thin Layer Agar - Evidence-based Tuberculosis Diagnosis - Tbevidence

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Procedure Manual Thin Layer Agar (TLA) Microcolony detection Rapid culture of Mycobacterium tuberculosis By Anandi MARTIN, PhD amartin itg.be & Juan Carlos PALOMINO, PhD palomino itg.be Institute.

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Lowenstein-Jensen (LJ) medium is most widely used for tuberculosis culture.

Culture remains the gold standard for laboratory confirmation of TB disease, and growing bacteria are required to perform drug-susceptibility testing and genotyping.

There are two kinds of tests used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests. A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria.

Drug susceptibility testing (DST) of Mycobacterium tuberculosis is generally carried out after a culture is isolated from a clinical specimen. This takes a long time, first to isolate a culture and then to perform drug susceptibility testing (indirect DST).

There are two kinds of tests used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests. A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria. It does not tell whether the person has latent TB infection (LTBI) or has progressed to TB disease.

Mycobacterial culture is the conventional gold standard for TB diagnosis. It is highly sensitive (limit of detection ~10–100 cfu/ml)5 but the time-to-result is lengthy (ranging from 2–8 weeks)6; moreover, the sample preparation is technically challenging, contamination prone and requires a BSL-3 facility.

Bacteriological confirmation of TB is necessary to test for resistance to first-line and second-line anti-TB drugs; such testing can be done using rapid molecular tests, phenotypic susceptibility testing or reference-level genetic sequencing ( 2).

Thin-layer agar method showed sensitivity and specificity values of 96.63% and 62.50%, respectively, for the isolation of Mycobacterium tuberculosis directly from specimens.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232