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GE Healthcare Instructions 52-1308-00 BB PD-10 Desalting Columns PD-10 Desalting Columns contains 30 prepacked disposable PD-10 Desalting Columns containing 8.3 ml of Sephadex G-25 Medium 4 adapters.

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The method is commonly referred to as desalting when the goal is to remove buffer salts from a sample in exchange for water (with water used to pre-equilibrate the gel-filtration resin). Buffer exchange is the term used when one set of buffer salt in a sample is exchanged for another set.

Desalting is used to remove salts from protein solutions, phenol or unincorporated nucleotides from nucleic acids or excess crosslinking or labeling reagents from conjugated proteins.

How many times can I reuse a Disposable PD-10 Desalting Column? Each column is intended for single use only.

Desalting columns use the principle of size exclusion chromatography, also called gel filtration chromatography. In addition to desalting, the other main use for size exclusion chromatography is size fractionation. The matrix beads in a size exclusion column contain pores.

Orobosa: PD-10 are pre-packed Sephadex G-25 resin for desalting and buffer exchange. It probably stands for "protein desalting". There are other brands of pre-packed desalting columns as well. You can also make them yourself with bulk Sephadex and empty columns made for the purpose.

HiPrep 26/10 Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.

In buffer exchange, the chromatography column is equilibrated with the final type of buffer salts. Either way, the salts within the solution will be replaced, either by water or a different buffer salt. With gel filtration, buffer salts and other small molecules within the substance will slide through the resin beads.

Desalting and buffer exchange use gel filtration chromatography (or size-exclusion chromatography) to separate soluble macromolecules from smaller molecules. When an aqueous solution is used to transport the sample through the column, the technique is referred to as gel-filtration chromatography.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232