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Protocol Pub. No. MAN0013443 Rev. A.0 Package contents Storage conditions Catalog Number 18090010 18090050 18090200 Size 2,000 units 10,000 units 4 10,000 units Protocol outline Kit Contents Store.

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Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

The SuperScriptâ„¢ IV First-Strand Synthesis System for RT-PCR is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The kit includes SuperScriptâ„¢ IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions.

We use an enzyme called "reverse transcriptase" to create a complementary DNA (cDNA) sequence from the RNA fragment. This creates hybrid molecules that are a combination of RNA and cDNA.

Prepare sample. RNA serves as the template in cDNA synthesis. ... Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. ... Select reverse transcriptase. ... Prepare reaction mix. ... Perform cDNA synthesis. ... Prepare sample. ... Remove genomic DNA. ... Select reverse transcriptase.

The useful range of input total RNA for the first strand cDNA template synthesis (reverse transcription) reaction is between 100ng and 5 µg.

Quantitative reverse transcription PCR (RT-qPCR) is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA).

First Strand cDNA Synthesis Protocols (E6300) Mix RNA sample and primer d(T)23VN in two sterile RNase-free microfuge tubes. Total RNA. ... Denature RNA for 5 minutes at 70°C. ... Add the following components to one tube. ... Incubate the 20 μl cDNA synthesis reaction at 42°C for one hour. ... Inactivate the enzyme at 80°C for 5 minutes.

The useful range of input total RNA for the first strand cDNA template synthesis (reverse transcription) reaction is between 100ng and 5 µg.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232