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TLC Visualization ReagentsThis is a brief selection of the many available TLC visualization reagents. Below each title is the type of compounds or structure which can be detected with the specific.

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Thin layer chromatography is an important tool for detecting amino acids by variety of spray reagents. Among these ninhydrin is the most popular due to its high sensitivity. However, ninhydrin produces the same purple/violet color with most amino acids.

Bromothymol blue is incorporated into the mobile phase as indicator and thus the acids appear as yellow spots on a blue background.

Preparation: Solution A: 1.7 g basic bismuth nitrate in 100 ml water/acetic acid (4:1). Solution B: 40 g potassium iodide in 100 ml of water. Mix reagents together as follows: 5 ml A + 5 ml B + 20 ml acetic acid + 70 ml water. Spray plates, orange spots develop.

However, if the substance is colourless, it may be treated with a reagent, which imparts it a characteristic colour. This reagent is given the name developer. Iodine is the most commonly used developer in paper chromatography. Several other techniques are available for locating the spots.

Vanadium (V) / sulfuric acid sulfuric acid.

Detection is performed by measuring absorbance of the column eluate with an ultraviolet detector at 280 nm, the absorbance wavelength for tyrosine and tryptophan, or at 214 nm, the absorbance wavelength for most amino acids and the peptide bond.

After the chromatogram has dried, one can identify the spots. If the spots can be seen, outline them with a pencil. Sometimes the color will fade over time, so the pencil outline is important. If the spots are not obvious, the most common visualization technique is to hold the paper under an ultraviolet lamp.

The most common non-destructive visualization method for TLC plates is ultraviolet (UV) light. A UV lamp can be used to shine either short-waved (254nm) or long-waved (365nm) ultraviolet light on a TLC plate with the touch of a button.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232