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  • Mlg 8.09 Isolation And Identification Of Listeria Monocytogenes From Red Meat

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United States Department of Agriculture Food Safety and Inspection Service Office of Public Health Science Laboratory QA/QC Division 950 College Station Road Athens, GA 30605 Laboratory Guidebook.

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The most common tools for Listeria testing are swabs and sponges. Swabs are typically used for food contact surfaces of tighter, hard to reach positions. Sponges are typically used for non-food contact surfaces such as walls and drains.

Current methods for identification of L. monocytogenes rely on physiological and biochemical methods. These include Gram stain morphology, catalase, motility, beta hemolysis on blood agar and oblique illumination of colonies on blood free agar (6).

monocytogenes involved the use of culture methods based on selective enrichment and plating. Isolates were identified by colony morphology, sugar fermentation and haemolytic properties. Genotypic identification of L. monocytogenes was performed using PCR incorporating 16S rRNA followed by DNA sequence analysis.

Isolation of the Listeria spp The selective media used for isolation of L. monocytogenes is Oxford agar. The media were ready as per the composition (Table 2). The culture (1ml) is taken from enrichment medium and streaked on the Oxford agar plates and incubated at 37°C for 48 hours.

The most common tools for Listeria testing are swabs and sponges. Swabs are typically used for food contact surfaces of tighter, hard to reach positions. Sponges are typically used for non-food contact surfaces such as walls and drains. Either can be used throughout the facility.

Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.

For optimal isolation of Listeria species, direct plating onto blood agar is recommended. This may be direct from clinical specimens or from subcultures of blood culture medium. In addition, when foodborne cases are being investigated, culture from faeces is often useful.

Isolation of the Listeria spp The selective media used for isolation of L. monocytogenes is Oxford agar. The media were ready as per the composition (Table 2). The culture (1ml) is taken from enrichment medium and streaked on the Oxford agar plates and incubated at 37°C for 48 hours.

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232