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How to fill out the DNOV052-Free T4-02032011 online
Filling out the DNOV052-Free T4-02032011 form is a straightforward process when approached step by step. This guide provides clear instructions to help users complete the form efficiently and accurately.
Follow the steps to fill out the DNOV052-Free T4-02032011 form online
- Click ‘Get Form’ button to access the DNOV052-Free T4-02032011 form and open it in your preferred document editor.
- Review the introduction section of the form to understand the purpose and importance of free T4 measurements in assessing thyroid function.
- Proceed to the 'Intended Use' section, ensuring you comprehend the competitive immunoenzymatic colorimetric method for free T4 determination, as described.
- Familiarize yourself with the 'Materials' provided in the form. Ensure you list all reagents needed for the assay, including anti-T4 antibodies and the T4-HRP conjugate.
- In the ‘Reagent Preparation’ section, follow the instructions closely for preparing various reagents, including the TMB substrate solution and wash solution.
- Collect your specimens as detailed in the 'Specimen Collection and Preparation' section. It is crucial to follow the specified guidelines for sample collection.
- Follow the detailed assay procedure. Pay special attention to incubation times and washing steps to ensure accuracy in results.
- In the 'Results' section, use the absorbance values recorded from the assay to calculate concentrations as instructed.
- Finally, review the quality control measures outlined in the form to ensure your assay performance remains reliable.
- After completing the form, you can save changes, download, print, or share the DNOV052-Free T4-02032011 document as needed.
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A low TSH and high T4 generally indicates hyperthyroidism. A high TSH and low T4 indicates primary hypothyroidism (due to a thyroid disease). A low TSH and low T4 suggest secondary hypothyroidism (due to a disease of the pituitary gland or hypothalamus of the brain).
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