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ExactSTART FullLength cDNA Library Cloning Kit Cat. No. ES0907 10 ReactionsConnect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/EpicentreBio), and Twitter ( EpicentreBio).

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How to fill out the ExactSTART Full-Length CDNA online

This guide provides step-by-step instructions for filling out the ExactSTART Full-Length CDNA Library Cloning Kit form online. Follow these instructions to ensure a smooth and successful completion of the form, tailored to your research needs.

Follow the steps to effectively complete the form

  1. Click the ‘Get Form’ button to access the ExactSTART Full-Length CDNA form and open it in the online editor.
  2. Review the introductory section to understand the purpose of the ExactSTART Full-Length cDNA Library Cloning Kit and its components.
  3. Fill in your personal information in the designated fields, ensuring that all entries are accurate and complete.
  4. Provide detailed information regarding the RNA sample to be used, including the total amount and quality indicators. Ensure you specify the type of RNA accurately.
  5. Complete the sections related to the reagents and additional required equipment, selecting those that you will be using in your cloning processes.
  6. Review any notes associated with each section, as they provide valuable information about the best practices for handling RNA and the inputs required for PCR amplification.
  7. Once you have completed the form, remember to save your progress periodically to avoid losing any information.
  8. After finalizing the form, users can choose to download a copy, print the document, or share it as needed.

Start filling out the ExactSTART Full-Length CDNA form online to advance your research!

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Full‐length cDNAs contain the complete sequence information of their respective mRNA templates. This information is determined by full‐length cDNA sequencing and provides knowledge of the protein encoded by the original mRNA and, in addition, of the untranslated regions (UTR) of this mRNA.

The synthesized cDNAs are of- ten shorter than the mRNA templates because of various processes that either degrade the mRNA or result in incom- plete transcription. (Note that reverse transcriptase is not made by human cells.

The 5′ untranslated region is the mRNA domain that contains plenty of elements such as upstream ORFs (uORFs), internal ribosome entry sites, microRNA binding sites, and structural components involved in the regulation of mRNA stability, pre-mRNA splicing, and translation initiation.

cDNA is derived from mRNA and contain the ORF as well as all or part of the 5' and 3' untranslated regions, to more closely mimic the context of native regulation.

The resultant mature mRNA, in eukaryotes, has a tripartite structure consisting of a 5' untranslated region (5' UTR), a coding region made up of triplet codons that each encode an amino acid and a 3' untranslated region (3' UTR).

cDNA (short for copy DNA; also called complementary DNA) is synthetic DNA that has been transcribed from a specific mRNA through a reaction using the enzyme reverse transcriptase. While DNA is composed of both coding and non-coding sequences, cDNA contains only coding sequences.

Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

The 5′ untranslated region (also known as 5′ UTR, leader sequence, transcript leader, or leader RNA) is the region of a messenger RNA (mRNA) that is directly upstream from the initiation codon.

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232