Get Clustalx

ClustalX is a powerful tool for performing multiple sequence alignments using a graphical user interface. This guide provides step-by-step instructions to help you navigate through the components of the ClustalX form and utilize its features effectively.

Follow the steps to fill out the ClustalX form online.

1. Press the ‘Get Form’ button to obtain the form and access it in the editor.
2. Start a ClustalX session by opening the application or program on your device. You will see a window with menu items at the top.
3. Load your sequences by selecting the 'Load Sequences' option from the File menu. A new window will appear for you to choose your sequences.
4. Highlight the file containing the unaligned sequences in the selection window and click the OK button. The sequences should now be displayed in the ClustalX window.
5. Select the desired output format by choosing the 'Output Format Options' from the Alignment menu. Close the window after making your selection.
6. Construct the alignment by selecting 'Do Complete Alignment' from the Alignment menu. This will initiate the alignment process of the loaded sequences.
7. Evaluate the alignment in the ClustalX window. Look for conserved positions and use the Quality menu for highlighting any poorly aligned segments.
8. If necessary, modify the alignment parameters by selecting 'Alignment Parameters' from the Alignment menu to optimize your results.
9. Rebuild the multiple alignment if you have changed the parameters using 'Do Alignment from Guide Tree' from the File menu.
10. To ensure quality, use the 'Show Low Scoring Segments' option under the Quality menu to identify unreliable sections.
11. Save your final alignment by selecting 'Save Sequences As' from the File menu, where you can choose a format and filename.
12. Optionally, create a PostScript image of your alignment by selecting 'Write alignment as PostScript' from the File menu.

Begin creating your multiple sequence alignments online with ClustalX today.