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  • Ascites Production Information Sheet

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HBPS-ASC-FRM-022 Effective Date: 20/MAY/2011 Issue: 02 Page 1 of 2 ASCITES PRODUCTION INFORMATION SHEET HARLAN BIOPRODUCTS FOR SCIENCE, INC. Researcher Name Customer PO # Company Name Size/Strain.

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How to fill out the ASCITES PRODUCTION INFORMATION SHEET online

The ASCITES PRODUCTION INFORMATION SHEET is a crucial document used in the production of ascitic fluid for research purposes. This guide provides clear and supportive step-by-step instructions to help users effectively complete the form online.

Follow the steps to successfully complete the ASCITES PRODUCTION INFORMATION SHEET.

  1. Click ‘Get Form’ button to obtain the form and open it in the editor.
  2. Enter your personal and contact information in the designated fields, including your name, company name, phone number, email address, and any customer PO number.
  3. Indicate the type of production you require: cGMP or research. Select the appropriate option based on your needs.
  4. Complete the 'Cell Line Information' section. Fill out the required fields marked with an asterisk, including the cell line designation and immunoglobulin species. Provide details about parental cells used in hybridoma production.
  5. Choose your media formulation by selecting one of the options provided. If using a custom formulation, indicate this in the 'Other' field.
  6. Provide information on growth conditions and whether the cell line has been exposed to any disease agents affecting humans.
  7. Outline the past history of ascites production with the specified cell line, including optimal cell dosage and expected yield.
  8. Confirm if alternatives to animal use have been considered and provide the source of the cell stock.
  9. In the 'Miscellaneous Information' section, indicate if IgG concentration is needed and specify any special production requirements.
  10. Detail the packaging requirements for clarified ascites by selecting your preferred option.
  11. Provide the shipping address for your cells and indicate whether you would like HBPS to destroy or ship back the cryostored cell line.
  12. Complete the billing and shipping addresses, including any required signatures and dates.
  13. Review the form for accuracy and completeness. Once you have filled out all sections, you can save changes, download, print, or share the form as needed.

Complete your ASCITES PRODUCTION INFORMATION SHEET online today for seamless processing.

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A typical monoclonal antibody production process Immunization of mice & isolation of splenocytes - Mice are immunized with an antigen and later their blood is screened for antibody production. The antibody-producing splenocytes are then isolated for in vitro hybridoma production.

Ascitic fluid (also called ascites) is an intraperitoneal fluid extracted from mice that have developed a peritoneal tumor. For antibody production, the tumor is induced by injecting hybridoma cells into the peritoneum, which serves as a growth chamber for the cells.

Ascites is the term that describes the fluid that fills the peritoneal cavity due to inflammation. For in vivo monoclonal antibody production, hybridoma cells are injected into the peritoneal cavity to resulting in ascites fluid containing the desired antibody.

There are two main methods used today to generate mAbs: (1) classical hybridoma fusion; and, (2) immune antibody libraries (Fig. 2 ). Mice have been very popular historically for mAb development due to the production of hybridoma derived mAbs.

The ascites method is one of the quickest for producing large amounts of monoclonal antibodies. One mouse can produce 3-5 ml of ascites fluid after undergoing several extraction cycles after inoculation. Inoculating multiple mice simultaneously can produce large batches of a monoclonal antibody.

Some of the commonly employed methods for the production of monoclonal antibody are: (1) fusion of sensitized lymphocytes and myelomas from different sources to produce continuous antibody-producing cell lines; (2) in vitro viral transformation of sensitized lymphocytes to form continuous antibody-producing cells; (3) ...

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Help Portal
Legal Resources
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232