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0.1M Citric acidSodium citrate buffer buffer pH range 3.0 6.2 Prepare a 0.1M solution of citric acid monohydrate, C6H8 O7H2O (21.01g/l) and a 0.1M solution of trisodium citrate dihydrate, C6H5 O7Na32H2O.

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The Citrate Buffer (pH 6.0), Concentrate (Cat. No. 005000) is a 20X solution and contains two compounds with different molecular weights, so there is no single molarity for the formulation. The citric acid is at about 20 mM and sodium citrate is at about 180 mM.

Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe Prepare 800 mL of distilled water in a suitable container. Add 24.269 g of Sodium Citrate dihydrate to the solution. Add 3.358 g of Citric Acid to the solution. Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).

Phosphate-citrate buffer is the buffer of choice for use with soluble horseradish peroxidase substrates such as o-phenylenediamine dihydrochloride (OPD) or tetramethylbenzidine (TMB). Phosphate-citrate buffer provides a pH of 5.0 and has good buffering capacity.

Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium.

Citrate Buffer (pH Range = 3.0 to 6.2) You can then adjust the final pH using a sensitive pH meter.

Buffer capacity (β) is defined as the moles of an acid or base necessary to change the pH of a solution by 1, divided by the pH change and the volume of buffer in liters; it is a unitless number. A buffer resists changes in pH due to the addition of an acid or base though consumption of the buffer.

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© Copyright 1997-2025
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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232