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Application NoteMicrosphere Coupling Twostep EDC/Sulfo NHS Covalent Coupling Procedure for Estapor Carboxylmodified Dyed Microspheres Introduction Proteins can be covalently coupled to the surface.

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CA DLSE-155 2003 CA e3067 2010 CA EDD DE 3BHW 2016 CA EDD DE 3DI-I 2018

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Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, however, decreases water- solubility of the modified carboxylate molecule, while activation with Sulfo-NHS preserves or increases water-solubility of the modified molecule, by virtue of the charged sulfonate group.

N-Hydroxysulfosuccinimide sodium salt (Sulfo-NHS) ≥98.0% (by HPLC, titration analysis) Description: CAS Number: 106627-54-7 MDL Number: MFCD00043100 Molecular Formula: C4H5NO6S Molecular Weight: 217.13 Purity/Analysis Method: 98.0% (HPLC,T) ...

Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution.

EDC should be stored at –20 °C in a desiccated container and allowed to equilibrate to room temperature before use. EDC is extremely sensitive to moisture and should not be used if damp or clumped.

EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O-acylisourea intermediate while allowing for efficient conjugation to primary amines at physiologic pH. Sulfo-NHS plus EDC (carbodiimide) crosslinking reaction scheme.

Activation using EDC and Sulfo-NHS is most efficient between pH 4.5 and 7.2. Therefore, it is often preferred to use MES (2-(N-morpholino) ethanesulfonic acid)) buffer at pH 6 for the activation reaction.

Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2).

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Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
DMCA Policy
About Us
Blog
Affiliates
Contact Us
Privacy Notice
Delete My Account
Site Map
All Forms
Search all Forms
Industries
Forms in Spanish
Localized Forms
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate workflows
DocHub
Instapage
Social Media
Call us now toll free:
1-877-389-0141
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232