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Application NoteMicrosphere Coupling Twostep EDC/Sulfo NHS Covalent Coupling Procedure for Estapor Carboxylmodified Dyed Microspheres Introduction Proteins can be covalently coupled to the surface.

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How to fill out the Microsphere Coupling Two-step online

This guide provides comprehensive instructions on filling out the Microsphere Coupling Two-step form online. By following the steps outlined below, users can ensure accurate completion of the form for successful protein coupling.

Follow the steps to complete your Microsphere Coupling Two-step form online.

  1. Click ‘Get Form’ button to obtain the form and open it in your document editor.
  2. Review the introductory information provided within the form to understand the purpose and significance of the two-step coupling protocol.
  3. Identify the relevant sections for activation using EDC and Sulfo-NHS. Fill in necessary fields regarding the volume and concentration of the reagents you plan to use, ensuring that all inputs match your experimental requirements.
  4. Detail the specifications for the microspheres being used, such as product numbers and the characteristics necessary for your experiment.
  5. Complete the fields pertaining to the conjugation process, including the concentration of antibodies and any special notes on the mixing and incubation parameters.
  6. Ensure that any troubleshooting information is filled out. Use guidelines provided within the document to make sure each section is completed based on common issues you may encounter.
  7. After filling out the entire form, make sure to review and make any necessary edits to guarantee all information is accurate before proceeding.
  8. Once confirmed, save your changes, and choose to download, print, or share the document as needed for your project.

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Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, however, decreases water- solubility of the modified carboxylate molecule, while activation with Sulfo-NHS preserves or increases water-solubility of the modified molecule, by virtue of the charged sulfonate group.

N-Hydroxysulfosuccinimide sodium salt (Sulfo-NHS) ≥98.0% (by HPLC, titration analysis) Description: CAS Number: 106627-54-7 MDL Number: MFCD00043100 Molecular Formula: C4H5NO6S Molecular Weight: 217.13 Purity/Analysis Method: 98.0% (HPLC,T) ...

Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution.

EDC should be stored at –20 °C in a desiccated container and allowed to equilibrate to room temperature before use. EDC is extremely sensitive to moisture and should not be used if damp or clumped.

EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O-acylisourea intermediate while allowing for efficient conjugation to primary amines at physiologic pH. Sulfo-NHS plus EDC (carbodiimide) crosslinking reaction scheme.

Activation using EDC and Sulfo-NHS is most efficient between pH 4.5 and 7.2. Therefore, it is often preferred to use MES (2-(N-morpholino) ethanesulfonic acid)) buffer at pH 6 for the activation reaction.

Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2).

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© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232
Form Packages
Adoption
Bankruptcy
Contractors
Divorce
Home Sales
Employment
Identity Theft
Incorporation
Landlord Tenant
Living Trust
Name Change
Personal Planning
Small Business
Wills & Estates
Packages A-Z
Form Categories
Affidavits
Bankruptcy
Bill of Sale
Corporate - LLC
Divorce
Employment
Identity Theft
Internet Technology
Landlord Tenant
Living Wills
Name Change
Power of Attorney
Real Estate
Small Estates
Wills
All Forms
Forms A-Z
Form Library
Customer Service
Terms of Service
Privacy Notice
Legal Hub
Content Takedown Policy
Bug Bounty Program
About Us
Blog
Affiliates
Contact Us
Delete My Account
Site Map
Industries
Forms in Spanish
Localized Forms
State-specific Forms
Forms Kit
Legal Guides
Real Estate Handbook
All Guides
Prepared for You
Notarize
Incorporation services
Our Customers
For Consumers
For Small Business
For Attorneys
Our Sites
US Legal Forms
USLegal
FormsPass
pdfFiller
signNow
airSlate WorkFlow
DocHub
Instapage
Social Media
Call us now toll free:
+1 833 426 79 33
As seen in:
  • USA Today logo picture
  • CBC News logo picture
  • LA Times logo picture
  • The Washington Post logo picture
  • AP logo picture
  • Forbes logo picture
© Copyright 1997-2025
airSlate Legal Forms, Inc.
3720 Flowood Dr, Flowood, Mississippi 39232